You are here

Article Text

Article menu
Download PDFPDF
Research Article
A simplified method for rapid determination of porphyrins by thin-layer chromatography.
  1. T Okuda,
  2. H Nakajima,
  3. K Yatsuki,
  4. M Amano,
  5. G Umeda

    Abstract

    Free porphyrins, especially copro-, uro-, and protoporphyrin, were successfully separated by talc thin-layer chromatography (TLC), and were determined from the relationship of the weight and the area of porphyrins in the spot on the thin-layer plate. The results obtained from both the solvent extraction and the simple, rapid TLC method reported in this paper showed good correlation for coproporphyrin levels up to 400 microgram/1. The TLC method was applied to the determination of urinary coproporphyrin in lead workers and in rats.

    https://doi.org/10.1136/oem.35.1.61

    Statistics from Altmetric.com

      Request Permissions

      If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.