Reference values for acoustic rhinometry are presented from 334 individuals without nasal symptoms between 4 years and 61 years old. There was a significant correlation between the minimal cross-sectional area (MCA) and nasal volume. Although the reproducibility of the method was good, the MCA varied widely. MCA correlated weakly to weight, height, age, and body mass index. Our data suggest that acoustic rhinometry is valuable for inter-individual comparisons.