Recent trends in biomonitoring of bisphenol A, 4-t-octylphenol, and 4-nonylphenol

Bisphenol A (BPA), 4-t-octylphenol (4-t-OP), and 4-nonylphenol (4-NP) are man-made alkylphenolic environmental contaminants possessing controversial endocrine disruption properties. Nowadays, an increased interest is raised for their accurate determination in biological media in order to estimate the exposure to these compounds and the associated health risk. The aim of this review is to present the available analytical methodologies for biomonitoring these three EDCs in human population. In non-occupational human exposure, they are detected in human matrices in trace level concentrations, commonly lower than 1ng/mL. The use of mass spectrometry based methods is particularly emphasized due to their well known superiority over sensitivity, selectivity and precision, even in difficult matrices, such as blood plasma and serum. Recent and most applicable sample preparation techniques are thoroughly presented. The benefits of solid phase extraction (SPE) and expected developments are demonstrated. Recent results from exposure assessment and epidemiologic studies for BPA, 4-t-OP and 4-NP are summarized and future trends are discussed.