Hepatitis E virus (HEV) is found in waste water which represents a route of infection. However, the risk for sewage workers is unknown. Thus, we aimed at determining the prevalence of anti-HEV antibodies in sewage workers from the Canton of Zurich. The anti-HEV antibodies evaluated in 288 subjects with exposure to sewage and 306 controls, a total of 594 subjects. Overall, twenty-two (3.7%) subjects had anti-HEV, the seroprevalence was similar in those exposed and controls. The most important risk factor, travelling in endemic areas, was lacking in two workers, who both were exposed to sewage. A follow up study in the population will soon give further evidence on the incidence rate.