Plant defense–related enzymes as latex antigens☆,☆☆,★,★★,♢
Section snippets
Sera
Sera from patients allergic to latex (sera nos. 1 to 15) were provided by PlasmaLab International (Everett, Wash.) and local hospitals in Japan. These patients had been diagnosed with latex allergy based on their clinical histories and positive IgE responses to crude latex proteins, which had been determined by a RAST. The sera from allergic subjects who had IgE antibodies to the crude latex proteins but did not claim definite subjective symptoms to latex products were classified as sera from
Separation and characterization of defense-related enzymes
The basic proteins that had not been adsorbed on an anion-exchange resin at pH 10.5 showed multiple bands on a native PAGE gel after silver staining. However, almost all of them were also detected on a zymogram for β-1,3-glucanase activity (data not shown). This indicated that the enzyme fraction was composed of multiple β-1,3-glucanase isoenzymes. When this enzyme fraction was analyzed by SDS-PAGE, three protein bands at 35, 36.5, and 38 kd were detected. Likewise, three broad peaks were
Discussion
In our continuing research on the relevance of plant defense–related proteins to latex allergy and the accompanying cross-reactivity,13, 19, 20, 26 the responsibilities of defense-related enzymes in NAL were investigated in this study. By combining several chromatographic procedures, we obtained three kinds of defense-related enzymes with sufficient purity for their antigenicity examination. Unfortunately, the number of the atopic subjects' sera used is too small to draw a definitive conclusion
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From aDivision of Medical Devices, National Institute of Health Sciences, Tokyo; bNiigata College of Pharmacy, Niigata; cNational Children's Hospital, Tokyo; and dUrafune Hospital, Yokohama City University, Yokohama.
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Supported in part by a grant from the Ministry of Health and Welfare, Japan.
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The views stated in this article are those of the authors and do not reflect the official policy of the Ministry of Health and Welfare, Japan.
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Reprint requests: Takeshi Yagami, MSc, Division of Medical Devices, National Institute of Health Sciences, Kamiyoga 1-18-1, Setagaya-ku, Tokyo 158, Japan.
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