Eosinophils, mast cells, and basophils in induced sputum from patients with seasonal allergic rhinitis and perennial asthma: Relationship to methacholine responsiveness☆,☆☆,★
Section snippets
Patients
We selected two groups of consecutive patients who were referred to our outpatient clinic: patients with a history of seasonal AR in response to grass pollen without asthma (AR group) and patients with AS (AS group).
Inclusion criteria for patients with rhinitis were: (1) a history of seasonal rhinitis without cough, wheezing, or shortness of breath during natural exposure, lasting 3 to 5 years; (2) positive skin test responses to grass pollens only; and (3) baseline FEV1 greater than 80% of
Results
Characteristics of patients with AR and asthma, who successfully accomplished sputum induction, are shown in Table I. We studied 30 patients with AR and 15 patients with asthma. Four patients in each group were former smokers. Seven patients with asthma were using inhaled β2-agonists as needed. The two groups were not different in regard to gender, age, and baseline FEV1. Methacholine PD20 values in the AR group were different from those of the AS group (p < 0.001, Table I). Patients in the AR
Discussion
In this study we demonstrated that eosinophils and metachromatic cells are present in induced sputum from patients with seasonal AR even outside exposure to the sensitizing antigen. In these patients, cell counts differed from those of patients with asthma. We found that metachromatic cells in induced sputum had the features of both mast cells and basophils. An important and new observation of this study was that basophils were present in seven of 30 (23%) sputum samples from patients in the AR
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2012, CytokineCitation Excerpt :But, the pathogenesis of BHR in AR patients is not clear. The proposed mechanisms for BHR in AR patients are the contraction of bronchial smooth muscle by mediators releasing from inflammatory cells reaching to the bronchi directly or by blood circulation and eosinophilic inflammation of the airways [4,16,17]. In the present study, BHR in SAR patients was not associated with blood eosinophil numbers, total IgE levels, and rhinitis severity during pollen season.
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From Servizio di Fisiopatologia Respiratoria, Modulo di Allergologia ed Immunopatologia Polmonare, Sesto San Giovanni, and Istituto di Clinica delle Malattie dell'Apparato Respiratorio, Università di Parma.
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Reprint requests: Antonio Foresi, MD, Servizio di Fisiopatologia Respiratoria, Viale Matteotti 83, 20099 Sesto San Giovanni, Italy.
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