The effect of low-level 60-Hz electromagnetic fields on human lymphoid cells: II. Sister-chromatid exchanges in peripheral lymphocytes and lymphoblastoid cell lines

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Abstract

Dividing human peripheral lymphocytes from 10 normal adults (5 males and 5 females) as well as lymphoid cell lines from patients with the chromosomal instability syndromes were exposed to low-level 60-Hz sinusoidal electromagnetic fields (EMF). The current density of the electrical field was 30 μA/cm2 while the strength of the magnetic field was either 1 or 2 gauss. The cytological endpoints measured included the frequency of sister-chromatid exchanges per chromosome; the distribution of first-, second-, and third-division cells and chromosome breakage (lymphoblastoid cells only). No statistically significant differences, indicative of EMF effects were observed between the treated and control cells regarding SCE frequency, cell cycle progression or chromosome breakage.

References (25)

  • P.S. Moorhead et al.

    Chromosome preparations from leukocytes cultured from human peripheral blood

    Exp. Cell Res.

    (1960)
  • S. Wolff

    Relation between DNA repair, chromosome aberrations, and sister chromatid exchanges

  • M.J. Andrews

    Induction of chromosome alignment by high frequency electric fields

    FEBS Lett.

    (1980)
  • M. Bauchinger et al.

    Analysis of structural chromosome changes and SCE after occupational long-term exposure to electric and magnetic fields from 380 kV systems

    Radiat. Environ. Biophys.

    (1981)
  • M.M. Cohen et al.

    Specificity of bleomycin-induced cytotoxic effects on ataxia telangiectasia lymphoid lines

    Cancer Res.

    (1983)
  • M.M. Cohen et al.

    Chemical clastogenicity in lymphoid cell lines of chromosomal instability syndromes

    Cancer Genet. Cytogenet.

    (1983)
  • M.M. Cohen et al.

    The effect of low level 60 Hz electromagnetic fields on human lymphoid cells. I. Mitotic rate and chromosome breakage in peripheral lymphocytes

    Bioelectromagnetics

    (1986)
  • D.W. Deno

    Currents induced in the human body by high voltage transmission line electric field-measurement and calculation of dose

    IEEE Trans. Power Appar. Sys. PAS

    (1977)
  • B. Greenbaum

    Effects of extremely low frequency fields on slime mold: Studies of electric, magnetic and combined fields, chromosome numbers and other tests

  • K. Hedner et al.

    Relationship between sister chromatid exchanges and structural chromosome aberrations in lymphocytes of 100 individuals

    Hereditas

    (1982)
  • J.A. Heller et al.

    A new physical method of creating chromosomal aberrations

    Nature (London)

    (1959)
  • W.T. Kaune et al.

    General properties of the interaction between animals and ELF electric fields

    Bioelectromagnetics

    (1981)

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