Short communicationA simple method of reducing the fading of immunofluorescence during microscopy
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2016, Coordination Chemistry ReviewsCitation Excerpt :Therefore, the ability to accurately and reliably detect pathologically relevant biomarkers would aid in the early diagnosis of human diseases, allowing for timely treatment, reduction in patient morbidity and improvement of quality of life. Conventional techniques for the sensing of macromolecular biomarkers include the enzyme linked immunosorbent assay (ELISA) [15,16], Western blotting [17], gel electrophoresis [18], immunofluorescence [19,20], polymerase chain reaction (PCR) [21], or flow cytometry [22,23]. For the detection of small molecular biomarkers, chromatographic techniques such as gas-liquid chromatography coupled to mass spectrometry are often employed [24].
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2015, Fungal Genetics and BiologyCitation Excerpt :The slide was then dipped in water to wash off the medium, immersed in fixative (9:1 ethanol to acetic acid) for 30 min at room temperature, flame-dried, and stored in a desiccator at room temperature until use. Slide specimens were stained with a mixture of DAPI (1 μg/ml) and propidium iodide (PI) (0.5 μg/ml) (hereafter, called DAPI/PI) dissolved in antifading mounting solution (Johnson and Araujo, 1981), then observed with an epifluorescence microscope (Olympus BH-2/BHS-RFC) and a 100× oil immersion objective lens (N.A 1.3). For fluorescence observation, either UV or G excitation or a triple band pass filter (D/F/R 612 BP405, Chroma Technology, Bellows Falls, VT) was used.
Flow cytometric applicability to evaluate UV inactivation of phytoplankton in marine water samples
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