Elsevier

Clinica Chimica Acta

Volume 59, Issue 2, 10 March 1975, Pages 227-232
Clinica Chimica Acta

The Jaffé reaction. Identification of the coloured species

https://doi.org/10.1016/0009-8981(75)90033-9Get rights and content

Abstract

Evidence is presented to show that the red species formed as the product of reaction between creatinine and alkaline picrate in the Jaffé reaction is a Janovsky complex.

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    Two considerations regarding sample collection should be highlighted. First, urinary creatinine levels were analyzed to account for any dilution of BTEX compounds in the urine according to the Jaffé reaction method (Butler, 1975). Second, none of the children provided first-time morning voids, and the circadian rhythm of urine production is expected to be similar in both groups since the time from waking up until urine collection was similar in both groups.

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