Skip to main content
Log in

Urinary malondialdehyde and 8-hydroxydeoxyguanosine as potential markers of oxidative stress in industrial art glass workers

  • Original Article
  • Published:
International Archives of Occupational and Environmental Health Aims and scope Submit manuscript

Abstract

Previous epidemiological studies have indicated that industrial art glass workers have increased mortality risks for certain types of cancer and for cardio-and cerebrovascular disease. To test the hypothesis that increased oxidative stress might contribute to these increased mortality risks, the urinary levels of the lipid peroxidation product, malondialdehyde (MDA), and the oxidative DNA adduct, 8-hydroxydeoxyguanosine (8OHdG) were determined in 343 workers (230 men and 113 women) from the art glass industry in the southeast of Sweden. Of the study subjects, 199 (181 men and 18 women) were engaged in the process of glass production and were regarded as exposed, whereas the remainders performed clerical, warehouse and other service work and were regarded as unexposed. One hundred and sixteen were smokers (75 men and 41 women) and 215 were non-smokers (142 men and 73 women). The findings indicate that (a) exposure to industrial art glass work per se does not cause any major oxidative stress as measured by urinary levels of MDA and 8OHdG, (b) the effects from smoking per se are limited to increased lipid peroxidation among men, and (c) joint exposure to industrial art glass work and smoking may cause increased lipid peroxidation among men and increased DNA hydroxylation among both men and women. While these findings provide no evidence for increased oxidative stress due to industrial art glass work per se, the increased 8OHdG excretion in workers who smoke may be associated with a higher risk of developing free radical-dependent degenerative disease including cancer

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Adelman R, Saul RL, Ames BN (1988) Oxidative damage to DNA: relation to species metabolic rate and life span. Proc Natl Acad Sci USA 85: 2706–2708

    Article  PubMed  CAS  Google Scholar 

  • Ames BN (1989) Endogeneous oxidative DNA-damage, aging, and cancer. Free Radic Res Commun 7: 121–128

    PubMed  CAS  Google Scholar 

  • Basu AK, Marnett LJ (1983) Unequivocal demonstration that malondialdehyde is a mutagen. Carcinogenesis 4: 331–333

    Article  PubMed  CAS  Google Scholar 

  • Cheng KC, Cahill DS, Kasai H, Nishimura S, Loeb LA (1992) 8-Hydroxyguanosine, an abundant form of oxidative DNA damage, causes G-T and A-C substitutions. J Biol Chem 267: 166–172

    PubMed  CAS  Google Scholar 

  • Cordioli G, Cuoghi L, Solari L, Berrino F, Crosignani P, Riboli E (1987) Mortalitá per tumore in una coorte di lavoratori della industria de vetro. Epidemiol Prev 30: 16–18

    Google Scholar 

  • Dean JA, Dean AG, Burton A, et al. (1988) EPI INFO. Division of Surveillance and Epidemiologic studies, Epidemiology Program Office, Centers for Disease Control, Atlanta, Georgia

    Google Scholar 

  • Dillard CJ, Tappel AL (1973) Fluorescent products from reaction of peroxidizing polyunsaturated fatty acids with phosphatidyl ethanolamine and phenylalanine. Lipids 8: 183–189

    Article  PubMed  CAS  Google Scholar 

  • Draper HH, Squires EJ, Mahmoodi H, Wu J, Agarwal S, Hadley M (1993) A comparative evaluation of thiobarbituric acid methods for the determination of malondialdehyde in biological materials. Free Radic Biol Med 15: 353–363

    Article  PubMed  CAS  Google Scholar 

  • Dubrow R, Wegman DH (1984) Occupational characteristics of cancer victims in Massachusetts: 1971–1973. DHHS (NIOSH) Publ. no. 84-109. National Institute for Occupational, Safety and Health, Cincinatti, Ohio

    Google Scholar 

  • Englund A, Ekman G, Zabrielski L (1982) Occupational categories among brain tumor cases recorded in the cancer registry in Sweden. Ann NY Acad Sci 381: 188–196

    Article  PubMed  CAS  Google Scholar 

  • Floyd RA (1990) The role of 8-hydroxydeoxyguanosine in carcinogenesis. Carcinogenesis 11: 1447–1450

    Article  PubMed  CAS  Google Scholar 

  • Floyd RA, Watson JJ, Wong PK, Altmiller DH, Rickard RC (1986) Hydroxyl free radical adduct of deoxyguanosine: sensitive detection and mechanism of formation. Free Radic Res Commun 1: 163–172

    PubMed  CAS  Google Scholar 

  • Fraga CC, Shigenaga MK, Park JW, et al. (1990) Oxidative damage to DNA during aging: 8-hydroxydeoxy-2′-deoxyguanosine in rat organ DNA and urine. Proc Natl Acad Sci USA 87: 4533–4537

    Article  PubMed  CAS  Google Scholar 

  • Frei B, Forte TM, Ames BN, Cross CE (1991) Gas phase oxidants of cigarette smoke induce lipid peroxidation and changes in lipoprotein protein properties in human blood plasma. Biochem J 277: 133–138

    PubMed  CAS  Google Scholar 

  • Goldstein B, Witz G (1990) Free radicals and carcinogenesis. Free Radic Res Commun 11: 3–10

    PubMed  CAS  Google Scholar 

  • González CA, Sanz M, Marco G, Pita S, Brullet E, Vida F, Agudo A, Hsieh C-C (1991) Occupation and gastric cancer in Spain. Scand J Work Environ Health 17: 240–247

    PubMed  Google Scholar 

  • Guichardant M, Valette-Talbi L, Cavadini C, Crozier G, Berger M (1994) Malondialdehyde measurement in urine. J Chromatogr B 665: 112–116

    Google Scholar 

  • Hall NEL, Rosenman KD (1991) Cancer by industry: analysis of a population-based cancer registry with an emphasis on bluecollar workers. Am J Ind Med 19: 145–159

    Article  PubMed  CAS  Google Scholar 

  • Halliwell B, Gutteridge J, Cross C (1992) Free radicals, antioxidants, and human disease: Where are we now? J Lab Clin Med 119: 598–620

    PubMed  CAS  Google Scholar 

  • International Agency on Research on Cancer (1993) IARC monographs on the evolution of carcinogenic risks to humans, vol. 58. Beryllium, cadmium, mercury, and exposures in the glass manufacturing industry. TARC, Lyon, France, pp. 347–375

    Google Scholar 

  • Kasai H, Nishimura S (1984) DNA damage induced by asbestos in the presence of hydrogen peroxide. Gann 75: 841–844

    PubMed  CAS  Google Scholar 

  • Kasai H, Crain PF, Kuchino Y, Nishimura S, Ootsuyama A, Tanooka H (1986) Formation of 8-hydroxyguanine moiety in cellular DNA by agents producing oxygen radicals and evidence for its repair. Carcinogenesis 1849–1851

  • Kiyosawa H, Suko M, Okudaira H, Murata K, Miyamoto T, Chung MH, Kasai H, Nishimura S (1990) Cigarette smoking induces formation of 8-hydroxydeoxyguanosine, one of the oxidative DNA damages in human peripheral leucocytes. Free Radic Res Commun 11: 23–27

    PubMed  CAS  Google Scholar 

  • Lagorio S, Tagesson C, Forastieri F, Iavoroni I, Axelson O, Carrere A (1994) Exposure to benzene and urinary concentrations of 8-hydroxydeoxyguanosine, a biological marker of oxidative damage to DNA. Occup Environ Med 1: 739–743

    Article  Google Scholar 

  • Leanderson P, Tagesson C (1989) Cigarette smoke potentiates the DNA damaging effect of man-made mineral fibres. Am J Ind Med 16: 697–706

    PubMed  CAS  Google Scholar 

  • Leanderson P, Tagesson C (1990) Cigarette smoke-induced DNA-damage: role of hydroquinone and catechol in the formation of the oxidative DNA-adduct, 8-hydroxydeoxyguanosine. Chem Biol Interact 75: 71–81

    Article  PubMed  CAS  Google Scholar 

  • Leanderson P, Tagesson C (1992) Cigarette smoke-induced DNA damage in cultured human lung cells: role of hydroxyl radicals and endonuclease activation. Chem Biol Interact 810: 197–208

    Article  Google Scholar 

  • Levin LI, Zheng W, Blot WJ, Gao Y-T, Fraumeni JF Jr (1988) Occupation and lung cancer in Shanghai: a case-control study. Br J Ind Med 45: 450–458

    PubMed  CAS  Google Scholar 

  • Loft S, Vistisen K, Ewertz M, Tjonneland A, Overvad K, Enghusen Poulsen H (1992) Oxidative DNA damage estimated by 8-hydroxydeoxyguanosine excretion in humans: influence of smoking, gender and body mass index. Carcinogenesis 13: 2241–2247

    Article  PubMed  CAS  Google Scholar 

  • Lynge L, Kurppa K, Kristofersen L, Malker H, Sauli H (1986) Silica dust and lung cancer: results from the Nordic occupational mortality and cancer incidence registers. J Natl Cancer Inst 77: 883–889

    PubMed  CAS  Google Scholar 

  • Malker HSR, McLaughlin JK, Weiner JA, Silverman DT, Blot WJ, Ericsson JLE, Fraumeni JF Jr (1990) Occupational risk factors for nasopharyngeal cancer in Sweden. Br J Ind Med 47: 213–214

    PubMed  CAS  Google Scholar 

  • Mallin K, Rubin M, Joo E (1989) Occupational cancer mortality in Illinois white and black males, 1979–1984, for seven cancer sites. Am J Ind Med 15: 699–717

    Article  PubMed  CAS  Google Scholar 

  • McConkey DJ, Orrhenius S (1994) Signal transduction pathways to apoptosis. Trends Cell Biol 4: 370–375

    Article  PubMed  CAS  Google Scholar 

  • McLaughlin JK, Malker HSR, Blot WJ, Malker BK, Stone BJ, Weiner JA, Ericsson JLE, Fraumeni JF Jr (1987a) Occupational risks for meningiomas of the CNS in Sweden. J Occup Med 29: 66–67

    PubMed  CAS  Google Scholar 

  • Milne R, Sandler DP, Everson RB, Brown SM (1983) Lung cancer and occupation in Alameda county: A death certificate casecontrol study. Am J Ind Med 4: 565–575

    Article  PubMed  CAS  Google Scholar 

  • Moffat RJ, Owens SG (1991) Cessation from cigarette smoking: changes in body weight, body composition and energy consumption. Metabolism 40: 465–470

    Article  Google Scholar 

  • Nilsson R, Nordlinder R, Ljungkvist G, Tagesson C, Walles S, Järvholm B (1996) Genotoxic effects in workers exposed to low levels of benzene from gasoline. Am J Ind Med (in press)

  • Pryor WA, Godbeer SS (1991) Non-invasive measures of oxidative stress status in humans. Free Radic Biol Med 10: 177–184

    Article  PubMed  CAS  Google Scholar 

  • Sankila R, Karjalainen S, Pukkala E, Oksanen H, Hakulinen T, Teppo L, Hakama M (1990) Cancer risk among glass factory workers: an excess of lung cancer? Br J Ind Med 47: 815–818

    PubMed  CAS  Google Scholar 

  • Shamberger RJ, Raymond J, Andreone TL, Willis CE (1974) Antioxidants and cancer. IV. Initiating activity of malonaldehyde as a carcinogen. J Natl Cancer Inst 53: 1771–1773

    PubMed  CAS  Google Scholar 

  • Shibutani S, Takeshita M, Grollman AP (1991) Insertion of specific bases during DNA synthesis past the oxidation damage base 8-oxodG. Nature 349: 431–434

    Article  PubMed  CAS  Google Scholar 

  • Shigenaga MK, Gemeno CJ, Ames BN (1989) Urinary 8-hydroxy-2′-deoxyguanosine as a biological marker of invo oxidative DNA damage. Proc Natl Acad Sci USA 86: 9697–9701

    Article  PubMed  CAS  Google Scholar 

  • Tagesson C, Källberg M, Leanderson P (1991) Determination of urinary 8-hydroxydeoxyguanosine by coupled-column high performance liquid chromatography with electrochemical detection: a non-invasive assay for in vivo oxidative DNA-damage in humans. Toxicol Methods 4: 242–251

    Google Scholar 

  • Tagesson C, Chabiuk D, Axelson O, Baranski B, Palus J, Wyszynska K (1993) Increased urinary excretion of the oxidative adduct, 8-hydroxydeoxyguanosine, as a possible early indicator of occupational cancer hazards in the asbestos, rubber and azo dye industries. Pol J Occup Med Environ Health 6: 357–368

    PubMed  CAS  Google Scholar 

  • Tagesson C, Källberg M, Klintenberg C, Starkhammar H (1995) Determination of urinary 8-hydroxydeoxyguanosine by automated coupled-column high performance liquid chromatography: a powerful technique for assaying in vivo oxidative DNA-damage in cancer patients. Eur J Cancer 31A, 6: 934–940

    Article  Google Scholar 

  • Wingren G, Axelson O (1985) Mortality pattern in a glass producing area in SE Sweden. Br J Ind Med 42: 411–414

    PubMed  CAS  Google Scholar 

  • Wingren G, Axelson O (1987) Mortality in the Swedish glassworks industry. Scand J Work Environ Health 13: 412–416

    PubMed  CAS  Google Scholar 

  • Wingren G, Englander V (1990) Mortality and cancer morbidity in a cohort of Swedish glassworkers. Int Arch Occup Environ Health 62: 253–257

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tagesson, C., Källberg, M. & Wingren, G. Urinary malondialdehyde and 8-hydroxydeoxyguanosine as potential markers of oxidative stress in industrial art glass workers. Int. Arch Occup Environ Heath 69, 5–13 (1996). https://doi.org/10.1007/BF02630732

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02630732

Key words

Navigation