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Urinary levels of 1-hydroxypyrene, 1-, 2-, 3-, and 4-hydroxyphenanthrene in females living in an industrial area of Germany

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Abstract

The concentrations of 1-hydroxypyrene (1-HOPYR), and 1-, 2-, 3-, and 4-hydroxyphenanthrene (HOPHE) as metabolites of pyrene and phenanthrene, were measured in urine samples collected from 124 housewives (27 smokers and 97 nonsmokers) living in Bottrop, an industrial city located in the Ruhr area in Germany. The urine samples were analyzed by a very sensitive and practical high-performance liquid chromatographic (HPLC) method using a two-column switching technique and a special precolumn packing material followed by fluorescence detection. The polycyclic aromatic hydrocarbon (PAH) metabolites are selectively enriched on the precolumn and separated from the matrix. Therefore, laborious clean-up steps were omitted. The above-mentioned PAH metabolites could be detected in all urine samples investigated. Smokers had significantly higher urine concentrations of 1-HOPYR (median 0.48 μg/g creatinine), 3-HOPHE (median 0.61 μg/g creatinine), 2-HOPHE (0.41 μg/g creatinine) and 4-HOPHE (median 0.10 μg/g creatinine) than non-smokers (median 0.15 μg/g creatinine, 0.31 μg/g creatinine, 0.31 μg/g creatinine and 0.04μg/g creatinine, respectively). The study shows that the influence of smoking is of such an order of magnitude that potential environmental exposure to PAH in this highly industrialized area is obscured by smoking habits. Furthermore, it can be concluded that the determination of 1-HOPYR, 1-, 2-, 3-, and 4-HOPHE in urine is a diagnostically useful method for the biological monitoring of persons environmentally exposed to PAH.

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Authors and Affiliations

  1. Institute and Clinic of Occupational, Social and Environmental Medicine of the University of Erlangen-Nürnberg, Schillerstraβe 25/29, D-91054, Erlangen, Germany

    J. Gündel, C. Mannschreck & J. Angerer

  2. Gesundheitsamt der Stadt Bottrop, Gladbecker Straβe 66, D-46215, Bottrop, Germany

    K. Büttner

  3. Hygiene Institute of the Ruhr Area, Rotthauser Straβe 19, D-45879, Gelsenkirchen, Germany

    U. Ewers

Authors
  1. J. Gündel
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  2. C. Mannschreck
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  3. K. Büttner
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  4. U. Ewers
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  5. J. Angerer
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Gündel, J., Mannschreck, C., Büttner, K. et al. Urinary levels of 1-hydroxypyrene, 1-, 2-, 3-, and 4-hydroxyphenanthrene in females living in an industrial area of Germany. Arch. Environ. Contam. Toxicol. 31, 585–590 (1996). https://doi.org/10.1007/BF00212444

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  • DOI: https://doi.org/10.1007/BF00212444

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