Abstract
Guinea-pigs were exposed to an aerosol of bacterial endotoxin (lipopolysaccharide, LPS). The free lung cell response and alveolar macrophage (AM) chemotaxis were studied. Neutrophils from guinea-pig blood gave larger migration responses than those obtained by intraperitoneal glycogen stimulation or human neutrophils. An increase in the number of neutrophils in the airways was found with a peak at 12–24 hours after exposure. In animals pre-treated with LPS inhalation for 4 months, the reaction was of shorter duration and smaller magnitude. AM showedin vitro chemotaxtic activity up to 4 hours after exposure; no difference was found in pre-treated animals. The results suggest that the neutrophil invasion in the airways after LPS is depedent on two mechanisms, the initial being AM chemotaxis, which is not modified by pre-exposure to LPS, and another unknown factor, which is modified by pre-exposure to LPS.
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Snella, MC., Rylander, R. Endotoxin inhalation induces neutrophil chemotaxis by alveolar macrophages. Agents and Actions 16, 521–526 (1985). https://doi.org/10.1007/BF01983657
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DOI: https://doi.org/10.1007/BF01983657