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Biological monitoring of workers exposed to N-N-dimethylformamide

II. Dimethylformamide and its metabolites in urine of exposed workers

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Abstract

N, N-Dimethylformamide (DMF) exposure was monitored in a synthetic leather factory; at the same time, urinary dimethylformamide and its metabolites were measured in urine samples collected before and at the end of workshifts. The study was run during two different periods. During the first phase ten workers were observed for 3 days (Monday, Tuesday and Wednesday) in the same week. In the second phase 16 workers were involved in the study on a Friday and on the following Monday. Urinary DMF, as well as hydroxymethyl-N-methylformamide and hydroxymethylformamide [measured as N-methylformamide (NMF) and formamide, respectively], were measured in all urine samples. Formamide was also measured as a “physiological” product in subjects not exposed to dimethylformamide. Environmental exposure to DMF ranged between about 10 and 25 mg/m3. The unmodified solvent found in urine collected at the end of the exposure was significantly related to the environmental concentrations of DMF; its urinary concentrations were found to range between 0.1 and 1 mg/l. Higher concentrations of NMF (mean 23.3 mg/1) and formamide (24.7 mg/1) were measured in urine samples collected at the end of workshifts. The same concentrations were related to individual exposures to DMF. N-Acetyl-S-(N-methylcarbamoyl)cysteine in the urine of workers exposed to DMF showed a mean concentration of 40.4 mg/1 on Friday (before and after the workshift) and a mean concentration of 10.3 mg/1 on Monday. Its slow kinetic profile favours its body accumulation during the working week. N, N-dimethylformamide and N-methylformamide in urine collected at the end of the workshift are good markers of current occupational exposure to dimethylformamide, while N-acetyl-S-(N-methylcarbamoyl)cysteine seems a useful biological marker for monitoring repeated exposure to the solvent.

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References

  1. American Conference of Governmental Industrial Hygienists (ACGIH) (1993) Threshold limit values for chemical substances and physical agents and biological exposure indices 1993–1994. ACGIH, Cincinnati

    Google Scholar 

  2. Barnes JR, Ranta KE (1972) The metabolism of dimethylformamide and dimethylacetamide. Toxicol Appl Pharmacol 23:271–276

    Article  PubMed  Google Scholar 

  3. Casal Lareo A, Perico A, Bavazzano P, Soave C, Perbellini L (1995) Biological monitoring of workers exposed to N,Ndimethylformamide. I Methods of analysis. Int Arch Occup Environ Health 67:41–46

    Google Scholar 

  4. Catenacci G, Ghittori S, Cottica D, Prestinoni A, Capodaglio E (1980) Occupational exposure to dimethylformamide and urinary excretion of monomethyl-formamide. G Ital Med Lav 2:53–57

    Google Scholar 

  5. Chary S (1974) Dimethylformamide, a cause of acute pancreatitis? Lancet II:356

    Google Scholar 

  6. Chivers CP (1978) Disulfiram effect from inhalation of dimethylformamide. Lancet 1:331

    Article  Google Scholar 

  7. Deutsche Forschungsgemeinschaft (1993) List of MAK and BAT values 1993. VCH, Weinheim

    Google Scholar 

  8. Ducatman AM, Conwill DE, Craw J (1986) Germinal tumors of the testicle among aircraft repairmen. J Urol 136:834–836

    Google Scholar 

  9. Kawai T, Tomojiro Y, Takao W, Cai S-X, Mei-Yuan H, LiQiang X, Jiang-Bin Q, Bang-Zong Y, Ikeda M (1992) Occupational dimethylformamide exposure. 2. Monomethyl-formamide excretion in urine after occupational dimethyl-formamide exposure. Int Arch Occup Environ Health 63:455–460

    Google Scholar 

  10. Kestell P, Gill MH, Threadgill MD, Gescher A, Howarth OW, Curzon EH (1987) Identification by proton NMR of N-(hydroxymethyl)-N-methyl formamide as the major urinary metabolite of N, N-dimethylformamide in mice. Life Sci 38:719–724

    Google Scholar 

  11. Kestell P, Threadgill MD, Gescher A, Gledhill AP, Shaw AJ, Farmer PB (1987) An investigation of the relationship between the hepatotoxicity and the metabolism of N-alkylformamides. J Pharmacol Exp Ther240:265–270

    PubMed  Google Scholar 

  12. Kimmerle G, Eben A (1975) Metabolism studies of N, Ndimethyl formamide. I. Studies in rats and dogs. Int Arch Arbeitsmed 34:109–126

    Google Scholar 

  13. Kimmerle G, Eben A (1975) Metabolism studies of N, Ndimethyl formamide. II. Studies in persons. Int Arch Arbeitsmed 34:127–136

    PubMed  Google Scholar 

  14. Krivanek ND, McLaughlin M, Fayerweather WE (1978) Monomethyl formamide levels in human urine after repetitive exposure to dimethyl formamide vapor. J Occup Med 20:179–182

    PubMed  Google Scholar 

  15. Lauwerys R, Kivits A, Lhoir M, Rigolet P, Houbeau D, Buchet JP, Roels HA (1980) Biological surveillance of workers exposed to dimethylformamide and the influence of skin protection on its percutaneous absorption. Int Arch Occup Environ Health 45:189–203

    PubMed  Google Scholar 

  16. Levin SM, Baker DB, Landrigan PJ, Monaghan SV, Frumin E, Braitwaite M, Towne W (1987) Testicular cancer in leather tanners exposed to dimethylformamide. Lancet II:1153

    Google Scholar 

  17. Maxfield ME, Barnes JR, Azar A, Trochimowicz MT (1975) Urinary excretion of metabolites following experimental human exposures to DMF or to DMAC. J Occup Med 17:506–511

    Google Scholar 

  18. Mraz J, Cross H, Gescher A, Threadgill MD, Flek J (1989) Differences between rodents and humans in the metabolic toxification of N, N-dimethyl formamide. Toxicol Appl Pharmacol 98:507–516

    Article  Google Scholar 

  19. Mraz J, Nohova H (1992) Percutaneous absorption of N, N-dimethyl formamide in humans. Int Arch Occup Environ Health 64:79–83

    Google Scholar 

  20. Mraz J, Nohova H (1992) Absorption, metabolism and elimination of N, N-dimethylformamide in humans. Int Arch Occup Environ Health 64:85–92

    Google Scholar 

  21. Mraz J, Turecek F (1987) Identification of N-acetyl-S-(N-methylcarbamoyl)-cysteine, a human metabolite of N, N-dimethyl formamide. J Chromatogr Biomed Appl 414:399–404

    Article  Google Scholar 

  22. Potter H (1973) Dimethylformamide induced abdominal pain and liver injury. Arch Environ Health 27:340–341

    Google Scholar 

  23. Sala C, Bernabeo F, Colombo G, Invernizzi E, Meneghel G (1984) Dimethylformamide risk. An evaluation in the production of artificial organic leather. G Ital Med Lav 6:143–148

    Google Scholar 

  24. Scailteur V, De Hoffman E, Buchet JP, Lauwerys R (1984) Study on in vivo and in vitro metabolism of dimethylformamide in male and female rats. Toxicology 29:221–234

    Article  Google Scholar 

  25. Scailteur V, Lauwerys R (1987) Dimethylformamide (DMF) hepatotoxicity. Toxicology 43:231–238

    Google Scholar 

  26. Wiles JS, Narcisse JK (1971) The acute toxicity of dimethylamides in several animal species. Am Ind Hyg Assoc J 32:539–545

    PubMed  Google Scholar 

  27. Yonemoto J, Suzuki S (1980) Relation of exposure to dimethylformamide vapor and the metabolite, methylformamide in urine of workers. Int Arch Occup Environ Health 45:159–165

    Google Scholar 

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Casal Lareo, A., Perbellini, L. Biological monitoring of workers exposed to N-N-dimethylformamide. Int. Arch Occup Environ Heath 67, 47–52 (1995). https://doi.org/10.1007/BF00383132

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