Table 2

Genome-wide significant associations between DNA methylation and exposure to pesticides stratified by gender, smoking status and by the presence of airway obstruction

								Low pesticide exposure			High pesticide exposure
CpG	Chr	Bp	Str	Gene	Island	Position	Mean beta (%)	B	SE	P	B	SE	P
Women (n=678)
cg10770187	2	150 186 923	−	LYPD6	Island	Intron; TSS200	5.90	−0.15	0.29	0.603	2.37	0.41	7.69×10⁻⁹
cg16884400	2	176 046 185	−	ATP5G3	Island	5′ UTR	6.41	0.01	0.23	0.953	1.74	0.32	4.10×10⁻⁸
cg08855288	8	63 161 589	−	NKAIN3	Island	5′ UTR	6.53	−0.32	0.23	0.169	1.69	0.32	1.47×10⁻⁷
cg23116540	15	39 871 808	−	THBS1	N_Shore	TSS1500	85.85	−0.43	0.50	0.386	−3.55	0.69	2.77×10⁻⁷
cg04256347	4	141 677 674	+	TBC1D9	Island	TSS1500	5.82	−0.24	0.17	0.160	1.23	0.24	4.04×10⁻⁷
Never-smokers (n=903)
cg03181524	19	39 048 014	+	RYR1	N_Shelf	Body	35.3	1.39	0.77	0.070	6.32	0.96	5.20×10⁻¹¹
Current-smokers (n=658)
cg07158503	5	135 415 693	−	VTRNA2-1	N_Shore	TSS1500	46.6	0.60	1.73	0.731	−13.20	2.55	2.24×10⁻⁷
cg14677130	5	38 445 916	−	EGFLAM	OpenSea	Body	85.8	0.64	0.91	0.485	−6.91	1.34	2.73×10⁻⁷
Subjects with airway obstruction (n=595)
cg15793258	19	4 638 503	−	TNFAIP8L1	N_Shore	TSS1500	61.1	0.15	1.22	0.904	−20.78	1.82	3.30×10⁻³⁰
cg25251562	2	3 704 773	−	ALLC	OpenSea	TSS1500	84.0	−2.75	1.08	0.011	−11.65	1.62	5.91×10⁻¹³
cg27484412	21	44 876 687	+	LINC00319	Island	3′ 3 kb	9.3	−0.28	0.26	0.281	2.12	0.39	4.17×10⁻⁸
cg15577272	7	157 932 397	+	PTPRN2	OpenSea	Body	82.5	−0.24	0.61	0.699	−4.87	0.91	9.31×10⁻⁸
cg12839813	16	89 114 720	−	CTD-2555A7.2	Island	Body	51.7	−0.92	1.02	0.367	8.07	1.53	1.25×10⁻⁷
cg10059942	10	124 908 488	+	HMX2	Island	Body	10.2	−0.24	0.37	0.517	2.80	0.55	2.91×10⁻⁷
cg17623116	3	26 751 066	+	LRRC3B	OpenSea	5′ UTR	83.7	−0.06	0.27	0.821	−1.98	0.40	8.84×10⁻⁷
cg20869844	7	140 103 726	−	RAB19	OpenSea	TSS200	9.2	0.04	0.31	0.900	2.20	0.46	1.29×10⁻⁶
cg12425700	4	75 186 779	−	BC016361	OpenSea	Body	77.4	−0.07	0.37	0.847	−2.64	0.55	1.46×10⁻⁶
cg26867890	6	39 692 938	+	KIF6	Island	Body	6.3	0.16	0.14	0.262	1.03	0.21	1.53×10⁻⁶
cg09961397	6	70 991 205	−	COL9A1	N_Shore	Body	79.6	−1.78	0.77	0.021	−5.51	1.15	1.55×10⁻⁶
cg03943218	10	102 419 600	+	PAX2	Island	5′ 85 kb	10.5	−0.18	0.27	0.509	1.90	0.40	2.11×10⁻⁶
cg20800022*	8	97 169 764	+	GDF6	Island	Body	12.8	0.32	0.31	0.312	−0.70	0.15	2.12×10⁻⁶
cg11465769	2	1 800 785	−	MYT1L	N_Shore	Body	77.4	−0.26	0.49	0.599	−3.46	0.73	2.18×10⁻⁶
cg10645314	2	3 704 589	−	ALLC	OpenSea	TSS1500	83.5	−5.23	1.71	0.002	−12.05	2.55	2.21×10⁻⁶
cg19084031	15	38 361 362	−	RP11-1008C21.2	N_Shelf	3′ 7 kb	18.3	0.09	0.70	0.904	4.97	1.05	2.23×10⁻⁶
cg04035638	6	31 141 768	+	PSORS1C3; POU5F1	OpenSea	Body (exon); body	85.3	−0.12	0.31	0.706	−2.19	0.47	2.51×10⁻⁶
cg19611616*	12	27 397 833	−	STK38L	S_Shore	5′ UTR	20.2	0.25	0.34	0.474	0.01	0.10	2.62×10⁻⁶
cg13082120	6	33 257 050	+	WDR46; PFDN6	S_Shore	1st exon; TSS1500	11.5	0.49	0.36	0.179	2.55	0.54	2.70×10⁻⁶
cg07508480*	2	46 496 100	+	EPAS1	OpenSea	5′ 28 kb	88.8	−0.02	0.24	0.934	−0.32	0.12	2.90×10⁻⁶

*Additionally adjusted for single nucleotide polymorphism (SNP) under the probe.
There were no significant associations between DNA methylation and pesticide exposure in the full cohort and in the stratified analysis in men or in individuals without airway obstruction.
B, beta; Bp, base pair; Ch, chromosome; CpG, DNA methylation site; Str, strand, UTR, untranslated region; TSS, transcription start site.