PT  - JOURNAL ARTICLE
AU  - Radu-Corneliu Duca
AU  - Matteo Creta
AU  - Katrien Poels
AU  - Lode Godderis
AU  - Jeroen Vanoirbeek
TI  - 855 Dermal exposure to diisocyanates: development and validation of an analytical method for accurately assessment of very low levels of exposure
AID  - 10.1136/oemed-2018-ICOHabstracts.1185
DP  - 2018 Apr 01
TA  - Occupational and Environmental Medicine
PG  - A415--A415
VI  - 75
IP  - Suppl 2
4099  - http://oem.bmj.com/content/75/Suppl_2/A415.1.short
4100  - http://oem.bmj.com/content/75/Suppl_2/A415.1.full
SO  - Occup Environ Med2018 Apr 01; 75
AB  - Introduction Dermal exposure to sensitizers such as diisocyanate have been described to promote the development of asthma in later stages when respiratory occupational exposure occurs. Therefore, we developed a reliable, sensitive and validated methodology based on dermal patches to assess skin exposure to diisocyanates.Methods An UPLC-Unispray-MS/MS method was established and validated in order to reach very low levels of detection. Custom-made dermal patches were developed in order to allow optimal sampling of diisocyanates. Their sampling capability was evaluated in a controlled environment test-chamber were patches were exposed to increasing concentrations of diisocyanates.Result The UPLC-MS/MS method using a Unispray ionisation source, based on supercritical fluids ionisation and Coanda effect, allowed reaching very low levels of detection (LoD=1 pg/mL) for all the targeted compounds (i.e. 4,4-MDI, 2,4-MDI, 2,6-TDI, 2,4-TDI, 1,6-HDI, and IPDI). Due to the high sensitivity of the analytical method, very low levels of diisocyanates (i.e. 25 pg/patch) are detected on the custom-made dermal patches. Furthermore, the patches allowed the sampling of a broad range of concentration levels (from 5 pg/cm2 to 5 ng/cm2), which have been correlated with the air levels from the controlled environment chamber-test.Discussion We succeeded to develop a method to assess dermal exposure to diisocyanates. Field studies are now necessary to further evaluate the suitability of the custom-made patches, as well as to relate low levels of exposure and potential health outcomes.