TY - JOUR T1 - 1204 Occupational exposure assessment to bioaerosols in poultry farming activities JF - Occupational and Environmental Medicine JO - Occup Environ Med SP - A193 LP - A193 DO - 10.1136/oemed-2018-ICOHabstracts.546 VL - 75 IS - Suppl 2 AU - DO Matuka AU - TS Singh AU - E Ratshikhopha AU - P Dayal AU - R Baatjies AU - D Ngajilo AU - MF Jeebhay Y1 - 2018/04/01 UR - http://oem.bmj.com/content/75/Suppl_2/A193.2.abstract N2 - Introduction Poultry farm workers are exposed to various hazardous biological agents in organic dust. Particulate dust, endotoxins and (1–3) β-d-glucans) in poultry dust have been implicated in adverse respiratory effects including work-related asthma.Method The study was conducted at various sites of a South African poultry farm consisting of broilers, rearing, laying, hatchery and catching processes. Personal inhalable samples were collected from 298 workers using filtration technique at a flow rate of 2 l/min for the entire work shift. Filter extracts were analysed for (1–3) β-d-glucan and endotoxin using the endpoint Glucatell and Limulus amebocyte lysate test (LAL) assays, respectively. Total inhalable dust concentration was also determined. Data were analysed using STATA 12.Results Dust particulate levels had an overall geometric mean (GM) of 11.04 mg/m3 and differed by farm process The mean dust particulate concentration was highest in the rearing department (GM=54.04 mg/m3, GSD=3.74) followed by hatchery (GM=36.25 mg/m3, GSD=3.46). The automated egg laying farms recorded elevated concentrations of dust particulate (GM=35.23 mg/m3), endotoxin (GM=880 EU/m3) and (1–3) β-d-glucan (GM=136 ng/m3) levels compared to the manual laying system (GM=3.95 mg/m3; GM=680 EU/m3; GM=97 ng/m3). Large broilers had relatively higher total dust particulate and endotoxin concentrations while small broilers had higher (1–3) β-d-glucan levels. The catching crew were found to have the highest concentrations of (1–3) β-d-glucan (GM=644 ng/m3, GSD=1.89) and endotoxin levels (GM=23902 EU/m3, GSD=9.64).Discussion This exposure characterisation study revealed that total inhalable dust, endotoxin and (1–3) β-d-glucans were very high and significantly variable between the five main poultry farming processes. Workers in all processes were exposed to endotoxin levels above the recommended OEL of 90 EU/m3. Inhalable dust particulate was also above the 10 mg/m3 OEL for total inhalable dust in the hatchery, rearing and automated laying farms. Poultry catchers appear to be at greatest risk of developing adverse respiratory health effects due to endotoxins and (1–3) β-d-glucans. ER -