TY - JOUR T1 - Wheat IgE profiling and wheat IgE levels in bakers with allergic occupational phenotypes JF - Occupational and Environmental Medicine JO - Occup Environ Med SP - 617 LP - 622 DO - 10.1136/oemed-2012-101112 VL - 70 IS - 9 AU - Mario Olivieri AU - Carlo Alberto Biscardo AU - Paola Palazzo AU - Sandra Pahr AU - Giovanni Malerba AU - Rosetta Ferrara AU - Danila Zennaro AU - Giovanna Zanoni AU - Luciano Xumerle AU - Rudolf Valenta AU - Adriano Mari Y1 - 2013/09/01 UR - http://oem.bmj.com/content/70/9/617.abstract N2 - Objectives To characterise occupational wheat allergic phenotypes (rhino-conjunctivitis, asthma and dermatitis) and immunoglobulin (IgE) sensitisation to particular wheat allergens in bakers. Methods We conducted clinical and immunological evaluations of 81 consecutive bakers reporting occupational symptoms using commercial tests (skin prick test (SPT), specific IgE, ISAC microarray) and six additional dot-blotted wheat allergens (Tri a 39, Tri a Trx, Tri a GST, Tri a 32, Tri a 12, Tri a DH). Results Wheat SPT resulted positive in 29 bakers and was associated with work-related asthma (p<0.01). Wheat IgE was detected in 51 workers and was associated with work-related asthma (p<0.01) and rhino-conjunctivitis (p<0.05). ISAC Tri a 30 was positive in three workers and was associated with work-related dermatitis (p<0.05). Wheat dot-blotted allergens were positive in 22 bakers. Tri a 32 and Tri a GST were positive in 13 and three bakers, respectively, and both were associated with work-related dermatitis (p<0.05). This association increased (p<0.01) when Tri a 32, Tri a GST and Tri a 30 were analysed together (p<0.01). Wheat IgE levels were associated with work-related dermatitis (p<0.01). Conclusions Wheat IgE levels and wheat microarrayed allergens may be associated with some occupational allergic phenotypes. The extension of the panel of wheat allergens may be promising for discriminating the clinical manifestations of baker's allergy. ER -