RT Journal Article SR Electronic T1 A cross-sectional study among detergent workers exposed to liquid detergent enzymes JF Occupational and Environmental Medicine JO Occup Environ Med FD BMJ Publishing Group Ltd SP 759 OP 765 DO 10.1136/oem.2008.045245 VO 66 IS 11 A1 F G B G J van Rooy A1 R Houba A1 N Palmen A1 M M Zengeni A1 I Sander A1 J Spithoven A1 J M Rooyackers A1 D J J Heederik YR 2009 UL http://oem.bmj.com/content/66/11/759.abstract AB Objectives: To investigate sensitisation and respiratory health among workers who produce liquid detergent products and handle liquid detergent enzymes.Methods: We performed a cross-sectional study among 109 eligible workers of a detergent products plant. 108 were interviewed for respiratory and allergic symptoms and 106 blood samples were taken from them to examine sensitisation to enzymes. Those sensitised to ⩾1 enzymes were referred for clinical evaluation. Workers and representatives were interviewed to characterise exposure qualitatively and estimate exposure semi-quantitatively. Workers were classified into three exposure groups with varying exposure profiles to enzymes, based on frequency, duration, and level of exposure.Results: Workers were exposed to proteases, α-amylase, lipase and cellulase. The highest exposures occurred in the mixing area. Liquid spills with concentrated enzyme preparations and leakage of enzymes during weighing, transportation and filling were causing workplace contaminations and subsequently leading to both dermal and inhalation exposure for workers.Workers with the highest exposures reported significantly more work-related symptoms of itching nose (prevalence ratio (PR) = 4.2, 95% CI 1.5 to 12.0) and sneezing (PR = 4.0, 95% CI 1.5 to 10.8) and marginally significant more symptoms of wheezing (PR = 2.9, 95% CI 0.9 to 8.7) compared with the least exposed group.Fifteen workers (14.2%) were sensitised to ⩾1 enzymes. A marginally statistically significant gradient in sensitisation across the exposure categories was found (p = 0.09). There was a clinical case of occupational asthma and two others with probable occupational rhinitis.Conclusions: Workers exposed to liquid detergent enzymes are at risk of developing sensitisation (14%) and respiratory allergy.