RT Journal Article SR Electronic T1 Stimulation of IL-8 release from epithelial cells by gas cooker PM10: a pilot study JF Occupational and Environmental Medicine JO Occup Environ Med FD BMJ Publishing Group Ltd SP 208 OP 210 DO 10.1136/oem.58.3.208 VO 58 IS 3 A1 C A J Dick A1 M Dennekamp A1 S Howarth A1 J W Cherrie A1 A Seaton A1 K Donaldson A1 V Stone YR 2001 UL http://oem.bmj.com/content/58/3/208.abstract AB OBJECTIVE To measure the effect of matter collected by a method that has a 50% efficiency for particles with an aerodynamic diameter of 10 μm (PM10), generated by gas and electric cooking, on A549 epithelial cells with and without nitrogen dioxide (NO2). METHOD Multiple indoor PM10 samples were collected on Teflon filters during the use of gas or electric cookers. Interleukin-8 (IL-8) concentrations were measured with a sandwich enzyme linked immunosorbent assay (ELISA) system. RESULTS Treatment of A549 cells with PM10 generated from gas cooking resulted in increased concentrations of IL-8 compared with untreated cells; particles from the electric cooker had no effect. NO2 did not alter the concentration of IL-8. CONCLUSION PM10generated by gas cooking has the potential to cause proinflammatory effects in lung cells. This may have implications for susceptible people.