@article {Nieboer56, author = {E Nieboer and S L Evans and J Dolovich}, title = {Occupational asthma from nickel sensitivity: II. Factors influencing the interaction of Ni2+, HSA, and serum antibodies with nickel related specificity.}, volume = {41}, number = {1}, pages = {56--63}, year = {1984}, doi = {10.1136/oem.41.1.56}, publisher = {BMJ Publishing Group Ltd}, abstract = {Serum from an individual with occupational asthma from nickel sulphate inhalation was shown by ammonium sulphate (Farr) and anti-IgG coprecipitation tests to contain antibodies which bind 63Ni2+. This binding was absent from 30 control sera. Ligand competition studies with serum and plasma from the propositus and controls are described, and include serial additions of HSA, L-histidine, EDTA, and the tripeptide gly-gly-L-his. The results, like those from metal ion blocking experiments, clearly showed that the formation of the reactive antigenic determinant depends on the selective binding of Ni2+ at the native Cu2+/Ni2+ transport site of HSA. Corroborating evidence is derived from the similar dependence on pH of the Ni2+-HSA formation and the ammonium sulphate 63Ni2+ coprecipitation reactions.}, issn = {0007-1072}, URL = {https://oem.bmj.com/content/41/1/56}, eprint = {https://oem.bmj.com/content/41/1/56.full.pdf}, journal = {Occupational and Environmental Medicine} }