Introduction The aim of the present study was to determine the association between shift work and chronic fatigue among Educational Hospital nurses.

Methods This cross-sectional study was carried out in an Educational Hospital in Yazd. Using stratified sampling technique, 200 Nurses were selected. The data was collected by using questionnaires about demographics and chronic fatigue. The data was analyzed using SPSS20 software and applying chi-square and Pearson correlation coefficients.

Results 57 male nurses and 143 female nurses participated in this study. There was no significant relation between the gender, marital status, work experience and education level with chronic fatigue. Between age groups with chronic fatigue there was a significant difference. Results from this study showed a significant relation between shift work and chronic fatigue. Chronic fatigue was less frequent among subjects with constant shifts than among those with non-constant shifts.

Conclusion The relation between shift work and chronic fatigue appears to differ according to constant and non-constant shifts.