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Prunus persica 9, a new occupational allergen from peach tree pollen involved in rhinitis and asthma
  1. Laura Victorio-Puche1,
  2. Natalia Pérez-Sánchez2,
  3. Maria Luisa Somoza3,
  4. Laura Martin-Pedraza4,
  5. Enrique Fernandez-Caldas5,6,
  6. Eva Abel Fernandez5,
  7. Mirian Moran5,
  8. Jose Luis Subiza5,
  9. Jose Damian Lopez-Sanchez7,
  10. Mayte Villalba8,
  11. Miguel Blanca3,
  12. José Antonio Cornejo-García9
  1. 1Allergy Department, Hospital General Universitario Morales Meseguer, Murcia, Spain
  2. 2Allergy Clinical Unit, Hospital Regional Universitario de Málaga, Málaga-IBIMA, Málaga, Spain
  3. 3Allergy Department, Hospital Universitario Infanta Leonor, Madrid, Spain
  4. 4Allergy Department, Fundación para la Investigación e Innovación Biomédica (FIIB) de los hospitales universitarios Infanta Leonor y Sureste, Madrid, Spain
  5. 5R&D Department, Inmunotek Laboratories, Madrid, Spain
  6. 6Division of Allergy and Immunology, University of South Florida College of Medicine, Tampa, Florida, USA
  7. 7Allergy Department, Hospital Clínico Universitario Virgen de la Arrixaca, El Palmar, Murcia, Spain
  8. 8Departamento de Bioquímica y Biología Molecular, Universidad Complutense de Madrid Facultad de Ciencias Químicas, Madrid, Comunidad de Madrid, Spain
  9. 9Instituto de Investigación Biomédica de Málaga-IBIMA, Málaga, Spain
  1. Correspondence to Dr Maria Luisa Somoza, Allergy department, Hospital Universitario Infanta Leonor, Madrid, Spain; mlsomoza{at}yahoo.com

Abstract

Objectives Several studies have described peach tree (PT) as an occupational allergen. The aim of this work was to assess the effect of Prunus persica 9 (Pru p 9), a recently identified allergen from PT pollen, in exposed workers.

Methods The study included people who reported respiratory symptoms after handling PT in orchards during the flowering period (Blanca village, Murcia region, south-east Spain). After completing a detailed questionnaire, participants underwent skin prick test (SPT) and nasal provocation test (NPT). The IgE response was analysed by SDS-PAGE and immunoblotting assays.

Results A total of 21 cases were included (mean age 45 years; 57% women). Most were polysensitised to common pollens, although one person was sensitised only to PT pollen. All cases had a positive SPT to this pollen, and 43% also to Pru p 9. All participants reported having rhinitis, and six participants reported having also asthma. Immunoblotting showed a heterogeneous IgE pattern for several proteins, with Pru p 9 recognised in nine cases. Most participants sensitised to PT pollen and Pru p 9 had positive NPTs, while those who were not sensitised to Pru p 9 tested negative.

Conclusions We demonstrate for the first time that Pru p 9, an allergen from PT pollen, can induce respiratory symptoms following occupational exposure. This must be considered a relevant allergen when people working with PT cultivars develop respiratory symptoms.

  • occupational health practice
  • allergy
  • occupational asthma
  • respiratory

Data availability statement

All data relevant to the study are included in the article or uploaded as supplementary information.

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Data availability statement

All data relevant to the study are included in the article or uploaded as supplementary information.

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Footnotes

  • LV-P and MLS contributed equally.

  • Contributors LVP: recruitment of cases, evaluation of cases, sample processing, and manuscript elaboration. NP-S: recruitment of cases, evaluation of cases, sample processing, and manuscript elaboration. MLS: evaluation of cases, undertaking of the nasal provocation tests, statistical analysis, and manuscript elaboration. LM-P: purification of Pru p, immunochemical studies, manuscript elaboration. EF-C: project design, allergenic extracts production, laboratory analysis, manuscript elaboration. EAF: allergenic extracts production, laboratory analysis, manuscript elaboration. MM: allergenic extracts production, laboratory analysis, manuscript elaboration. JLS: project design, manuscript supervision and elaboration. JDL-S: recruitment of cases, evaluation of cases, sample processing, and manuscript elaboration. MV: project design, manuscript supervision and evaluation. MB: project elaboration, patient recruitment and evaluation, skin testing for molecular allergens, data management, and manuscript elaboration. JAC-G project elaboration, data management, and manuscript elaboration.

  • Funding JAC-G was recipient of grant PI15/01317 and MB was the recipient of grant PI17/00615, both supported by the Institute of Salud Carlos III, NHS, Spain. All authors were supported by the collective grant of Networks RIRAAD and ARADyAL.

  • Competing interests None declared.

  • Provenance and peer review Not commissioned; externally peer reviewed.

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