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Original research
Decrease in serum testosterone levels after short-term occupational exposure to diisononyl phthalate in male workers
  1. Jean-Bernard Henrotin1,
  2. Eva Feigerlova2,
  3. Alain Robert3,
  4. Mathieu Dziurla1,
  5. Manuela Burgart3,
  6. Anne-Marie Lambert-Xolin3,
  7. Fanny Jeandel3,
  8. Georges Weryha2
  1. 1 Occupational Epidemiology Division, National Research and Safety Institute, Vandoeuvre-Les-Nancy, France
  2. 2 Department of Endocrinology, University Hospital of Nancy, Vandoeuvre-Les-Nancy, France
  3. 3 Toxicology and Biometrology Division, National Research and Safety Institute, Vandoeuvre-Les-Nancy, France
  1. Correspondence to Dr Jean-Bernard Henrotin, Occupational Epidemiology Division, National Research and Safety Institute, Vandoeuvre-Les-Nancy 54519, France; jeanbernardhenrotin{at}wanadoo.fr

Abstract

Objective The objective of the study was to examine the effects of occupational exposure to diisononyl phthalate (DINP) on serum testosterone levels in male workers.

Methods From 2015 to 2018, 97 male workers were recruited from six French factories in the plastics industry. In a short longitudinal study, changes over 3 days in the level of total or free serum testosterone and DINP exposure were measured. DINP exposure was measured by urinary biomonitoring: mono-4-methyl-7-oxo-octyl phthalate (OXO-MINP), mono-4-methyl-7-hydroxy-octyl phthalate (OH-MINP) and mono-4-methyl-7-carboxyheptylphthalate (CX-MINP). We further analysed changes in follicle-stimulating hormone, luteinising hormone, total testosterone to oestradiol ratio and two bone turnover markers (procollagen-type-I-N propeptide, C terminal cross-linking telopeptide of type I collagen), and erectile dysfunction via standardised questionnaires (International Index of Erectile Function, Androgen Deficiency in Aging Males). Linear mixed models were used with the variables ‘age’ and ‘abdominal diameter’ included as confounder.

Results Increased urinary OXO-MINP was associated with a significant decrease in total serum testosterone concentrations, but only for workers who exhibited the smallest variations and lowest exposures (p=0.002). The same pattern was observed for CX-MINP but was not significant; no association with OH-MINP was detectable. More self-reported erectile problems were found in workers exposed directly to DINP at the workstation (p=0.01). No changes were observed for the other biological parameters.

Conclusions Short-term exposure to DINP is associated with a decrease in total serum testosterone levels in male workers. Our results suggest that DINP could present weak antiandrogenic properties in humans, but these need to be confirmed by other studies.

  • hygiene / occupational hygiene
  • occupational health practice
  • endocrine disorders
  • exposure assessment
  • male reproduction
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Footnotes

  • Contributors J-BH: conception and study design, acquisition, statistical analysis, interpretation of data, drafting and revising the manuscript. EF: conception, interpretation of data, drafting and revising the manuscript. AR: conception, biochemical analyses, interpretation of data, drafting and revising the manuscript. MD: data manager, statistical analysis. MB, A-ML-X, FJ: biochemical analyses, interpretation of data. GW: conception, interpretation of data, drafting and revising the manuscript for important intellectual content. All authors approved the final manuscript.

  • Funding This work was supported by funding from the National Research and Safety Institute.

  • Competing interests None declared.

  • Patient consent for publication Not required.

  • Ethics approval The study was approved by the French regional committees of medical research ethics of the East III region (N° 2014-A01506-41) in accordance with national legislation. Furthermore, written informed consent was obtained from each participant.

  • Provenance and peer review Not commissioned; externally peer reviewed.

  • Data availability statement No data are available.

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