Introduction Oxidative stress leads to many kinds of diseases. Various chemicals in workplaces induce oxidative stress. In addition, psychological stress also increases oxidative stress. To prevent diseases potentiated by oxidative stress, a method for the appropriate assessment of the oxidative stress status is needed. Currently, urinary 8-hydroxydeoxyguanosine (8-OHdG) is widely measured as an oxidative stress biomarker. There is a specific advantage if saliva can be used as the sample to measure the oxidative stress biomarker, because saliva is much easier to collect than urine. However, the accurate measurement of 8-OHdG in saliva is impractical, because the quantity of 8-OHdG in saliva is quite low. In this study, we investigated the measurement of 8-hydroxyguanine (8-OHGua) in saliva, as an oxidative stress marker.
Methods The 8-OHGua levels in saliva were analysed by a column switching HPLC system equipped with an electrochemical detector (HPLC-ECD).
Results The 8-OHGua in saliva could be detected as a single peak by HPLC-ECD. The salivary 8-OHGua levels of smokers were significantly higher than those of non-smokers.
Conclusion Salivary 8-OHGua may be a useful biomarker in the human population, in relation to the assessment of the oxidative stress induced by various factors in working environments.
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