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Exposure assessment
Wood dust exposure and risk of lung cancer
  1. Parveen Bhatti1,
  2. Laura Newcomer1,
  3. Lynn Onstad1,
  4. Kay Teschke2,
  5. Janice Camp3,
  6. Michael Morgan3,
  7. Thomas L Vaughan1
  1. 1Program in Epidemiology, Division of Public Health Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA
  2. 2School of Environmental Health, University of British Columbia, Vancouver, British Columbia, Canada
  3. 3Department of Environmental and Occupational Health Sciences, School of Public Health, University of Washington, Seattle, Washington, USA
  1. Correspondence to Parveen Bhatti, Program in Epidemiology, Division of Public Health Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA; pbhatti{at}fhcrc.org

Abstract

Objectives Despite the compelling association between wood dust and sinonasal cancer, there has been little systematic and rigorous study of the relationship between wood dust and lung cancer. We investigated whether a history of wood dust exposure through occupational and hobby-related activities was associated with increased lung cancer risk.

Methods We conducted a population-based case-control study, with 440 cases and 845 age-matched controls. Using detailed work and personal histories, quantitative estimates of cumulative exposure to wood dust (thought to be primarily from softwood) were calculated for each participant. Using unconditional logistic regression adjusted for age and smoking status, risk of lung cancer was examined in relation to employment in wood-related occupations, working with wood as a hobby, as well as cumulative wood dust exposure that took into account both occupational and hobby-related sources.

Results While we observed an increased risk of lung cancer associated with working in a sawmill (OR=1.5; 95% CI: 1.1, 2.1), we found no evidence of increased risks with other occupations, working with wood as a hobby or with estimated cumulative exposure to wood dust. Contrary to our hypothesis, we observed modest decreased risks with exposure to wood dust, although no dose-response relationship was apparent.

Conclusions This study provided somewhat reassuring evidence that softwood dust does not increase the risk of lung cancer, but future studies should evaluate exposure to hardwood dusts. Suggestive evidence for an inverse association may be attributable to the presence of endotoxin in the wood dust, but the lack of a dose-response relationship suggests a non-causal relationship.

  • Epidemiology
  • cancer
  • wood dust

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What this paper adds

  • Results from previous studies of occupational wood dust exposure and lung cancer have been mixed.

  • We constructed an exposure metric that accounted for intensity, frequency and duration of exposure to wood dust based on detailed work history data.

  • We found suggestive evidence of an inverse association between wood dust and lung cancer risk.

  • This study provides somewhat reassuring evidence that softwood dust does not increase the risk of lung cancer.

Introduction

Dust from the processing of wood is one of the more commonly encountered hazardous agents. More than 600 000 persons in the USA are occupationally exposed on a regular basis, and many more come in contact with wood dust through hobbies.1 Operations performed on wood can create dust in two ways: by shattering wood cells and by chipping out whole cells.2 Shattering, which occurs almost exclusively during sanding operations, for example, results in a finer particle size and higher dust concentration than does chipping, which is more likely to occur in sawing and milling operations.3 4 However, it is clear from industrial hygiene sampling that dust particles of respirable size are produced from even the coarsest wood processing activities.4

Clinical observations in the 1960s in the High Wycombe area near Oxford, England suggested that workers involved in the manufacture of furniture from hardwoods experienced substantially increased risks of sinonasal adenocarcinoma,5 later estimated to be approximately 500-fold higher compared to the general population.6 A subsequent study of this cancer occurring in England and Wales outside of the Oxford area confirmed a high RR (RR 95) nationwide for adenocarcinoma among furniture workers and also observed a fivefold increased RR among other woodworkers, principally carpenters and joiners, compared to the general population.7 While sinonasal cancer has been clearly associated with hardwood dust, increased risks with softwood dust have not been ruled out.1 For instance, a recent study of occupation and cancer among 15 million people in five Nordic countries found that sinonasal cancer risk was increased among workers who were exclusively exposed to softwood dust.8 Furthermore, studies in cell lines have shown both hardwood and softwood dusts induce inflammation responses, generate reactive oxygen species and induce DNA damage,9–11 all of which are recognised as important mechanisms in carcinogenesis.

Wood dust has long been recognised as a respiratory irritant, with studies of dust from certain tree species demonstrating adverse effects on the lung, including asthma, chronic obstructive pulmonary disease, decline in lung function over the workshift, and decreased forced vital capacity, among woodworkers in a variety of jobs.12–14 Results of studies examining associations between wood dust and lung cancer, however, have been inconclusive,1 15–21 which may be attributable to the relatively crude exposure assessments that have been used in many of these studies. Thus, the aim of the present case–control study was to investigate the possible association between wood dust exposure and lung cancer, with individual quantitative estimates of wood dust exposure, in the more rural areas of Washington State where work in wood-related industries and wood-related hobbies are common.22–24

Methods

Procedures for this study were approved by the Fred Hutchinson Cancer Research Center Institutional Review Board, and all participating subjects provided informed consent.

Identification of cases and controls

Cases were prospectively identified through rapid reporting mechanisms of the Fred Hutchinson Cancer Research Center's Cancer Surveillance System, a population-based registry that operates as part of the Surveillance, Epidemiology and End Results program of the National Cancer Institute. Eligible cases included all men who were newly diagnosed with lung cancer between 1 May 1993 and 31 July 1996 and who, at the time of diagnosis, were between 18 and 74 years of age and resided in an 11-county area of western Washington, where work in wood-related industries is most common. The two most populous counties that include the cities of Seattle and Tacoma were excluded because wood-related occupations were expected to be rare in these areas. All eligible cases were selected for the study with the exception of those who were 70–74 years of age at diagnosis, of whom 50% were randomly selected; given the natural rise of lung cancer incidence with age, this was done to limit the relatively large number of older participants who may have had difficulty recalling details of their occupational history.

Controls were males identified between 1993 and 1996 from the same geographical area as the cases through random-digit dialling, using a modified Waksberg's method,25 and were frequency matched to cases by 5-year age group. In this method, a representative sample of households in a geographical region is identified by the random generation of telephone numbers from lists of working exchanges. Each number is called up to nine times at different times of the day and week over a period of up to several months to see whether the number identifies a residence, and if so, whether an eligible control resides there.

Data collection

Experienced interviewers conducted structured telephone interviews with cases and controls. Of 929 eligible cases identified by the cancer registry, telephone interviews were successfully completed for 821 (88.4%). To maintain comparability with controls, 37 cases without a telephone at the time of diagnosis were excluded, yielding 784 cases for the study. Clinical information regarding the cancer, including histological type and subsite, was determined from medical records by trained registry personnel.

In ascertaining controls, 8129 residential phone numbers were identified, of which 7870 (96.8%) were successfully called to determine if an eligible control resided in the household. A total of 1047 eligible controls were identified and approached for interview, of whom 883 (84.3%) were successfully interviewed, yielding an overall control response rate of 81.6%. Seven controls without a residential telephone 1 year before the interview were excluded, leaving 876 for the study.

If a subject died before the interview could be arranged, or was too ill, a proxy respondent (usually the wife) was interviewed. This occurred for 344 case and 31 control interviews. Given that proxies were unlikely to accurately recall detailed information regarding work histories, cases and controls requiring proxy respondents were excluded from the current analysis to minimise the impact of exposure misclassification. This left 440 cases and 845 controls for analysis.

All questions in the interview referred to the period before the reference date, which was 1 year before diagnosis for cases and 1 year before ascertainment for controls. Cases were interviewed a median of 129 days after diagnosis, while controls were interviewed a median of 48 days after ascertainment. We collected information on demographic background, previous medical conditions, family history of cancer, use of tobacco and alcohol products, and a lifetime occupational and chemical exposure history. For each job that a respondent held for at least 6 months, we enquired about the job title, typical activities and duties in the job, the type of industry, and the start and stop dates.

Most of the interview focused on specifics of employment in seven wood-related industries: logging, sawmills, plywood manufacturing, particle board manufacturing, pulp and paper mills, finish carpentry and general carpentry. For each industry in which a participant had worked, we enquired about involvement in processes specific to that industry which were known to usually entail significant exposure to wood dust. For each such process we collected information on the participant's usual activities related to the process, the duration and typical hours per day spent in such activities, and a number of potential modifiers of exposure. For example, for those who had worked in the plywood industry, we enquired about working near where the wood was sawn, where it was shaped (ie, planed, routed or moulded), where it was chipped and where it was sanded, and use of compressed air to clean dusty areas. For certain processes, for example, sanding, we further enquired about distance from the wood dust source (the sander) and use of enclosed booths. A set of questions also focused on working with wood as a hobby, and collected data on duration and frequency of specific activities including sawing, sanding and shaping, and the usual location (outdoors versus indoors).

Exposure assessment

Estimation of exposure to wood dust was based on an algorithm that took into account the typical concentration specific to the occupational and hobby-related process involved and a number of modifiers of that concentration. The typical exposure levels and modifiers were based on a detailed review of the published industrial hygiene literature,26–29 examination of databases containing representative measurements of wood dust concentrations linked with specific processes and field measurements carried out specifically for this study.

Wood dust data were requested from the Occupational Safety and Health Administration Integrated Management Information System (OHSA IMIS) for the period from its inception (1 May 1979) to 31 July 1997.30 The database included 1632 measurements taken by state and federal inspection personnel and ancillary data about the industry, occupation, location of the work site, wood type and circumstances of measurement. Dust concentrations were measured using a non-specific ‘total’ particulate technique,31 although wood dust would be expected to predominate in these measurements.

Worksite sampling of 8 h total wood dust concentrations targeted several industries for which we judged the existing literature and data were limited or unreliable, including the chip screening areas and chipping yards in the pulp and paper industry, the logging and yarding areas of the timber industry, and various operations of a door manufacturing and cabinet making plant. The purpose of these measurements was to estimate typical dust concentrations in occupations and industries judged to be less well represented in the OSHA database.

The processes considered, typical exposure levels for each process and applicable industries are listed in table 1. Activities in the logging industry were judged not to entail significant wood dust exposures and were therefore not included in the estimates of exposure. Table 2 describes the modifiers of exposure that were assessed, the processes they were applicable to, and the appropriate multiplier associated with each factor level. For example, an operator of a power sander in the finish carpentry industry, who was typically within 1.5 m of the tool, would be assigned a concentration of 19 mg/m3 for the exposure period (100% of the unmitigated level), whereas someone who typically worked near (1.5–3.0 m) a sanding operation would be assigned 30% of the typical exposure (5.7 mg/m3).

Table 1

Wood dust concentrations by process for specific industries and hobby-related woodwork

Table 2

Modifiers of wood dust concentration

For each process, each individual's cumulative exposure to wood dust (in units of months-mg/m3) was calculated by summing the product of duration (months) working in or near the process, the proportion of a 40 h work week typically spent in or near the process (this was calculated for hobbies as well), the average exposure intensity (mg/m3) and the modifying factors.

Statistical analyses

RRs were estimated by calculation of ORs and 95% CIs using unconditional logistic regression models. We examined RR of lung cancer in relation to employment history in wood-related occupations and processes and history of wood-related hobbies, as well as in relation to categories of cumulative wood dust exposure from individual occupational and hobby-related processes and to all processes combined. Quartiles of cumulative wood dust exposure were calculated based on the distribution of cumulative wood dust exposure among controls.

Age (≤39, 40–49, 50–59, 60–69, ≥70 years) and smoking status (never, former, current) were included as covariates in all models. Race, education and pack-years of smoking were also examined as potential confounding factors but did not have a material impact (≥10% change) on the point estimates of interest and so were not included in the final analyses. Analyses were repeated for specific histological subtypes (carcinoma not otherwise specified, large cell, small cell, squamous, adenocarcinoma, other). Possible interactions between smoking and wood dust exposure were examined by stratum-specific analyses, and by evaluating interaction terms in the logistic model.

Results

Demographic characteristics of the cases and controls are summarised in table 3. Cases and controls were quite similar in age distribution, with mean ages of 61.8 and 61.1 years, respectively. Over 95% of subjects were classified as Caucasian, with controls reporting more years of education than cases. As expected, a larger proportion of cases were current smokers and had greater pack-years of smoking than controls. In terms of general work history, controls tended to report working a greater number of jobs than cases; however, cases and controls reported similar total years worked and ages at first job. For cases, histological classification and disease stage are also listed in table 3. The most common histological groups were adenocarcinoma, squamous cell and small cell carcinoma.

Table 3

Demographic and disease characteristics of lung cancer cases and controls

Table 4 describes the history of working in wood-related jobs and hobbies in lung cancer cases and controls. There was statistically significant evidence of an increased risk of lung cancer among those reporting working in sawmills, whereas working as a general carpenter and working with wood as a hobby were associated with modestly, but significantly, decreased risks. Working in a plant that manufactured plywood, in a plant that manufactured particle board and in a pulp and paper mill were also associated (non-significantly) with modestly decreased risks.

Table 4

Wood-related jobs of lung cancer cases and controls

The distribution of estimates of cumulative exposure to wood dust from all sources and from each primary process are summarised in table 5. Overall, there was no evidence of increased lung cancer risk associated with cumulative exposure to wood dust. Many of the RR estimates were below unity, although none were statistically significant, and no dose–response was evident. Stratification by histological type or smoking status did not produce markedly different results (not shown).

Table 5

Process specific and total exposure to wood dust of lung cancer cases and controls

Discussion

In this population-based case–control study, we found no evidence of increased risks of lung cancer when evaluating wood dust exposure in occupational and hobby-related settings in Washington State. In fact, contrary to our hypothesis, we observed slightly decreased risks with exposure to wood dust in most specific processes as well as overall. However, most of the effect estimates were not statistically significant, and no dose–response relationship was apparent. We also observed very little evidence of increased risk associated with specific wood-related jobs or working with wood as a hobby, with the exception of a 50% increased risk associated with working in a sawmill.

Of the 24 lung cancer case–control studies reviewed by the International Agency for Research on Cancer (IARC)1 (no cohort studies were identified), only seven specifically assessed exposure to wood dust. Of these, four studies provided evidence of slightly increased risks. The inconsistent findings and lack of exposure–response relationships led the IARC working group to conclude that there was no indication of a role for occupational exposure to wood dust in the causation of lung cancer. More recent studies have also produced mixed results, but, unlike our study, a lack of data accounting for intensity, frequency and duration of exposure to wood dust has been a major limitation of these studies.15–21

Over a 6-year follow-up in the American Cancer Society Prevention Study-II, there was an increased risk of lung cancer among men reporting exposure to wood dust (OR 1.2; 95% CI 1.0 to 1.3).21 However, there was no evidence of an increasing risk of lung cancer with increasing duration of exposure except when the analysis was restricted to woodworkers. A large US based case–control study (1368 cases, 1192 controls) found that a history of work in a wood dust-related occupation or industry (based on self-reported longest employment in a wood-related occupation or industry, or self-reported exposure to wood dust from a list of substances) was associated with an increased risk of lung cancer (OR 3.2; 95% CI 1.5 to 6.9).17 Similar risks were observed across the various histological subtypes and there was evidence of a trend with increasing years of employment in these occupations or industries and evidence of an increased effect with smoking. In another US based case–control study (809 cases, 1522 controls), individuals reporting 20 or more years of ‘regular’ exposure to occupational wood dust were at an increased risk of developing lung cancer (OR 2.2; 95% CI 1.3 to 3.6).19 Similar risks were seen across the various histological subtypes, except for large cell lung cancer. There was also suggestive evidence of effect modification by smoking status, with increased risks of wood-dust related lung cancer observed among smokers but not among non-smokers. While the exposure assessment accounted for frequency, respondents were asked to report it as ‘usually’, ‘occasionally’, ‘never’ or ‘don't know’ without any definition of the categories. In a cohort study of furniture workers in Estonia, there was no evidence of increased incidence of lung cancer compared to the general population,18 and an increased risk was not observed when evaluating ‘moderate’ or ‘high’ exposure jobs or when considering various durations of employment. A recent occupational cohort study used a job–exposure matrix to evaluate cumulative exposure to a variety of organic dusts and also found no evidence of increased lung cancer risk with increasing exposure to wood dust.20 While the study used quantitative exposure data, exposure levels were assigned based on job titles rather than subject-specific occupational histories.

Strengths of the present study include its population-based design, the large sample size based in a geographical area with a relatively large concentration of wood workers and detailed exposure assessment that included field measurements. We were able to account for the intensity, frequency and duration of exposure to various occupational sources of wood dust, which has been a major limitation of previous studies.

A limitation of our study is that exposure estimates were based on recalled occupational histories and working conditions. Differential recall may explain controls reporting a greater number of jobs than cases, and thus the protective effects of exposure that were observed. However, cases and controls reported similar numbers of years worked and ages at first job, and because exposures were not assigned based on self-reported wood dust exposures, but rather the specific job and tasks performed by participants were assigned exposures via an objective protocol, recall bias is less likely. Residual confounding from incomplete adjustment for smoking may be a concern; however, this is unlikely to explain the observed protective effects since smoking status and pack-years were found to be uncorrelated with cumulative wood dust exposure. Another limitation is that a large number of cases could not be interviewed directly because they had died or were too ill. Although we obtained work history data from proxy respondents for these cases, we excluded them from analysis to minimise the impact of exposure misclassification. While our results may be biased if exposure levels for the excluded cases were systematically different, these two groups of cases were not significantly different in terms age, race, smoking history and cancer histology (results not shown). Cases with proxy respondents were, however, more likely to be diagnosed with distant stage disease (66%) as compared to directly interviewed cases (33%).

While epidemiological studies have more clearly established the carcinogenicity of hardwood dust (in association with sinonasal cancer) than that of softwood dust, recent studies in cell lines have shown softwood dusts to induce carcinogenic mechanisms such as inflammation, production of reactive oxygen species and DNA damage in cell lines.9–11 Nonetheless, a limitation of this study, as with many others, is the lack of data on wood types that participants worked with. In Washington softwoods are primarily used in industrial activities; however, exposure to hardwood dust cannot be ruled out. We were also unable to account for differences in the fraction of respirable dusts between the various processes, since exposure estimates were based on measurements of total dust. However, no major differences were observed in effect estimates when stratified by process type.

The suggestive evidence of an inverse association, in part, may be attributable to the presence of endotoxin in wood dust,32 exposure to which has been observed in sawmills and joineries.33 34 Endotoxin has been associated with a decreased risk of lung cancer in other occupational settings, particularly the textile industry.35 36 Interestingly, while a recent study among Shanghai textile workers found decreased risks of lung cancer attributable to endotoxin,35 a report from the same cohort found increased risks of sinonasal cancer,37 making an inverse association of lung cancer with wood dust exposure somewhat plausible. However, because we observed an increased risk of lung cancer among sawmill workers and did not observe a relationship of decreasing lung cancer risk with increasing wood dust exposure, this suggests the absence of a protective effect. Furthermore, exposures to endotoxin among woodworkers are much lower than those observed for textile workers. For example, assuming that 1 ng of endotoxin is equivalent to 10 endotoxin units (EU),38 the geometric mean of inhalable endotoxin measured during a 6–8 h work shift across various sawmills and joineries in Australia ranged from 3.7 to 91.1 EU/m3.34 Across various processes in three textile factories in Shanghai, the geometric mean of inhalable endotoxin measured during half-shift periods ranged from 40.3 to 2079.2 EU/m3.35

Overall, we did not observe an increased risk of lung cancer with increased exposure to wood dust. Rather, we found suggestive evidence that wood dust exposure was associated with a decreased risk of lung cancer. A protective effect has not been observed in previous studies, but exposure assessment in many of these studies was a major limitation. The lack of a dose–response relationship with cumulative exposure suggests a non-causal relationship, but future studies exploring the possible role of exposure to endotoxin in wood dust in the aetiology of lung cancer may be fruitful.

References

Footnotes

  • Funding Support for this research was provided by grant R01CA53392 from the National Cancer Institute (USA).

  • Competing interests None.

  • Ethics approval This study was conducted with the approval of the Fred Hutchinson Cancer Research Center Institutional Review Board.

  • Provenance and peer review Not commissioned; externally peer reviewed.

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