Article Text
Abstract
Background: Poisson regression is routinely used for analysis of epidemiological data from studies of large occupational cohorts. It is typically implemented as a grouped method of data analysis in which all exposure and covariate information is categorised and persontime and events are tabulated.
Aims: To describe an alternative approach to Poisson regression analysis using single units of persontime without grouping.
Methods: Data for simulated and empirical cohorts were analysed by Poisson regression. In analyses of simulated data, effect estimates derived via Poisson regression without grouping were compared to those obtained under proportional hazards regression. Analyses of empirical data for a cohort of 138 900 electrical workers were used to illustrate how the ungrouped approach may be applied in analyses of actual occupational cohorts.
Results: Using simulated data, Poisson regression analyses of ungrouped persontime data yield results equivalent to those obtained via proportional hazards regression: the results of both methods gave unbiased estimates of the “true” association specified for the simulation. Analyses of empirical data confirm that grouped and ungrouped analyses provide identical results when the same models are specified. However, bias may arise when exposureresponse trends are estimated via Poisson regression analyses in which exposure scores, such as category means or midpoints, are assigned to grouped data.
Conclusions: Poisson regression analysis of ungrouped persontime data is a useful tool that can avoid bias associated with categorising exposure data and assigning exposure scores, and facilitate direct assessment of the consequences of exposure categorisation and score assignment on regression results.
 regression models
 cohort studies
 statistical methods
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Poisson regression is a method of modelling disease rates as a function of covariate levels that is often applied in the analysis of data from occupational cohort studies.^{1} Analyses are typically conducted using grouped input data in the form of a tabulation of persontime and events in which all predictor variables are categorised.^{2–}^{5} Although categorisation of an exposure indicator is sometimes criticised, it remains useful, and in some circumstances even preferable to analyses of exposure data in continuous form.^{1,}^{6,}^{7} However, for the purpose of estimating quantitative exposureresponse relations, categorisation of exposure data that were originally measured on a continuous scale often leads to loss of power and questions about the sensitivity of study findings to decisions about exposure categorisation and score assignment.^{7,}^{8,}^{9,}^{10}
One way to address concerns about the consequences of exposure categorisation is to utilise a regression method, such as Cox proportional hazards regression, that accommodates continuous data.^{2,}^{11} However, proportional hazards regression methods can be extremely intensive computationally for analyses of large occupational cohorts. This is particularly true for analyses involving interactions and time dependent variables; in such cases Cox regression models may fail to converge.^{11}
In this paper we describe how Poisson regression analyses using single units of persontime, rather than the standard grouped persontime approach, may be used to directly evaluate these concerns. This ungrouped approach avoids the need to categorise variables originally measured on a continuous scale and facilitates examining the influence on regression results of exposure categorisation and score assignment. The researcher can use the same regression model and methods applied for analyses of grouped data, but without categorisation of predictor variables. In addition, Poisson regression allows the rate ratio, a fundamental epidemiological indicator, to be estimated directly from the data. We illustrate the ungrouped Poisson regression method and its application through simulations and analyses of empirical data from a large occupational cohort.
METHODS
Poisson regression
The assumption of a classical Poisson regression model is that the number of events in a particular unit of time follows the Poisson distribution with a mean nλ, where for observation i the rate λ_{i} is related to a vector of independent explanatory variables, X_{i,} by
where β is a vector of unknown parameters to be estimated and n_{i} represents the time at risk and is equivalent to the rate denominator. The quantity log(n_{i}) is often referred to as the “offset” of the model. With this model, a cohort is typically crossclassified by levels of exposure and other predictor variables, X_{i}, and the time at risk is calculated for each of the resulting combinations of X.
Ungrouped input structure for persontime data
In order to conduct Poisson regression analysis of ungrouped persontime data, an analytical data set is constructed in which there is a unique observation for each unit of persontime at risk. An example of the analytical data for one subject from a simulated cohort (described in detail below) is shown in table 1. One subject contributes multiple observations, and the total number of observations is equal the total personyears of follow up. As indicated in column 2 of the table, a binary indicator of case status is associated with each observation. The indicator is assigned a value of “0” for each observation (each unit of persontime at risk) until the date of last observation; at that point, a value of “1” is assigned to cases (and a value of “0” is assigned to noncases). As indicated in column 3, each observation represents one unit of persontime (in this example, one year of follow up). Therefore, all observations contribute equal weight and the offset term need not be specified when fitting the Poisson regression model to ungrouped data.
Main messages

Poisson regression models can be fit to ungrouped persontime data, as well as to input data in the traditional, tabular form.

With ungrouped input data, exposure need not be categorised, but can instead be expressed as a continuous, quantitative variable.

Ungrouped Poisson and Cox regression models give equivalent results, but Poisson regression directly estimates rate ratios and may have advantages in computational efficiency.

The ungrouped approach can avoid bias associated with exposure categorisation.

Poisson regression models based on grouped and ungrouped data provide identical estimates of exposuredisease association and precision when the models are equally specified.
As shown in columns 4–5 of table 1, each observation can be associated with independent predictor variables that are measured on a continuous scale. Column 4 illustrates how each unit of persontime is associated with an attained age (measured on a continuous scale). Column 5 shows how each observation is also associated with a cumulative exposure level.
Simulation
Hypothetical data were generated for 100 cohorts, each with 25 000 workers. At the start of follow up, each simulated worker was assigned an ageatentry into the cohort, maximum lengths of follow up and employment, and an exposure rate equal to the amount of exposure accumulated in one year (table 2). The distribution of ageatentry and lengths of follow up and employment are similar to those observed in a study of nuclear industry workers.^{12} The median age at entry is 25 years, while the 90th centile for ageatentry is 41 years. The median lengths of employment and follow up are 17 years and 35 years, respectively. For each personyear of observation contributed by a simulated subject, disease status was determined by calculating the probability of disease under the model:
where δ_{0} and δ_{1} are parameters for a Weibull model centred at age 55 years that defines the age specific probability of disease in the absence of exposure, where x and age are time dependent indicators representing cumulative exposure and the natural logarithm of attained age, respectively, and φ is the effect of exposure on the probability of disease. We allowed for censoring of observations because of death due to causes other than the one under investigation by calculating, for each personyear, the age specific probability of censoring via the model:
where c is the probability of censoring, and η_{0} and η_{1} are parameters of a Weibull model that defines the age specific risk of the death due to causes other than the one under investigation. Values of δ_{0}, δ_{1}, η_{0}, η_{1}, and φ are given in table 2. Further details of the simulation methods used here (and an example of the SAS code used to generate simulated cohort data) are given in a previous publication.^{13}
Policy implications

Ungrouped Poisson regression may be a preferred approach for risk assessment.
Empirical data
Data for empirical analyses were obtained from a retrospective study of mortality among a cohort of 138 905 male electrical workers in the United States. Details of the study, which was originally designed to examine the risk of leukaemia and brain cancer in relation to exposure to magnetic fields, have been presented elsewhere.^{14} Briefly, the men were employed for at least six months at any of five electric power companies between 1950 and 1986 and were followed through 1988, yielding 20 733 deaths. Exposure to 60 Hz magnetic fields was estimated by linking individual work histories with quantitative data derived from 2842 fullshift personal magnetic field measurements.^{15,}^{16} The large size and unusually complete follow up (97%) of this cohort make it particularly useful for methodological research. For the purpose of the current analysis, we considered the association of brain cancer with exposure to magnetic fields, estimated as unlagged cumulative exposure in micro Teslayears (μTy). Note that the risk estimates obtained here are not necessarily equal to those published previously because of differences in parameterisation and model specification.
Data analysis
Poisson regression models were fit to the simulated and empirical data. The simulated data were entered in ungrouped form, as described above, and no offset term was specified. Age and exposure were the only explanatory variables in analyses of simulated data.
Poisson regression analyses of empirical data from the electrical workers cohort were conducted with the input data entered both in ungrouped form and in the classical, tabular form. When the tabular input form was used, all of the predictor variables, including exposure, were categorised and an offset term was included in the model. Quantitative exposure scores for categorical analyses were assigned by dividing the data at deciles of the exposure distribution among all personyears or among personyears of brain cancer cases only, and then selecting the mean exposure level of each category to represent the exposure of all events and persontime at risk in that category. We also considered scores based on category midpoints. However, we showed in a previous paper^{10} that midpoint exposure scores tend to increase the bias resulting from categorisation, so in the interest of brevity those data are not shown here. When the ungrouped form of input was used, exposure was also entered as a continuous variable. Models fit to the cohort data were adjusted for age and calendar time, which were categorised in 10 year increments when grouped and ungrouped models were compared. Race (in two categories) was considered as an additional predictor in some analyses to approximate the complexity of typical occupational cohort analyses.
Proportional hazards regression was also used to derive estimates of cumulative exposuremortality trends using the simulated and empirical data. Attained age was specified as the timescale to obtain relative risk estimates. Cumulative exposure was treated as continuous variable and, in analyses of empirical cohort data, calendar time and race were included as additional explanatory variables to match the Poisson regression models.
The SAS system (SAS Institute, Cary, North Carolina, USA) was used to generate the simulated cohorts, compute persontime at risk, and fit the regression models.
RESULTS
Estimates of the exposuredisease association in simulated data were obtained using proportional hazards regression and Poisson regression analyses of ungrouped persontime data. Analyses by both methods yielded quantitatively similar results, as indicated in fig 1 by the alignment of the estimates along a line of equality. The average estimate via each method was 0.40 (diamond in fig 1), equal to the true magnitude of association specified in the simulation.
Parallel analyses of the occupational cohort data using proportional hazards regression and Poisson regression with ungrouped input data also yielded identical estimates of the exposuredisease association and its standard error (table 3).
To evaluate the effect of exposure categorisation and score assignment, Poisson regression models were also fit to grouped persontime data from the occupational cohort. In the grouped data, cumulative exposure was represented by exposure scores based on mean values for categories defined by deciles of the exposure distribution among all personyears or deciles of the exposure distribution among cases. Estimates of the association based on categorised exposure were different from those obtained with a continuous exposure variable and ungrouped Poisson regression or proportional hazards regression (table 3), suggesting that results obtained with the categorical approach are biased. The apparent bias was reduced by using the distribution of exposure among cases, rather than among all personyears, as the basis for categorisation (table 3).
We also fit identically specified Poisson regression models to the empirical cohort data in tabular and ungrouped form. This required that exposure and all covariates be categorised, because the tabular input form cannot accommodate continuous variables. These models yielded identical estimated RRs and 95% confidence intervals for both forms of input (data not shown).
DISCUSSION
We propose that Poisson regression analysis of ungrouped persontime data can be used to estimate quantitative exposureresponse relations and address concerns about potential bias resulting from the definition of exposure variables. This approach allows Poisson regression models to be applied to occupational cohort data with exposure estimates entered as a continuous variable. As a result, it facilitates the comparison of alternative forms of exposureresponse, ranging from categorisation to parametrically and nonparametrically smoothed curves.^{7,}^{17} It also permits the investigator to use the same regression method, and in fact the identical regression models, to examine the same data, in an ungrouped format, that are analysed in classical Poisson regression in a tabular form. To our knowledge, the ungrouped approach to Poisson regression has not been described previously, although it may have been applied in a recent analysis of occupational cohort data.^{18}
It has been asserted that the Poisson regression method is equivalent to the risk set approach of Cox proportional hazards regression under the situation in which each cell of the crossclassification of persontime and events includes a single event.^{1,}^{11} This assertion is correct for the situation in which estimates of association are derived solely using categorical variables. However, if scores are assigned to exposure categories in order to estimate doseresponse trends, then these approaches are not equivalent. Even if each cell of the persontime table includes a single death it may include multiple personyears at risk. The score assigned to that cell will not necessarily provide an unbiased estimate of the true exposure for the persontime and the decedent in each cell. In contrast, the ungrouped approach we describe, in which each unit of persontime and each event is associated with its measured exposure, will in fact converge to the risk set approach to analyses of continuous data as units of persontime become increasingly small. We have shown through simulation and analyses of empirical occupational cohort data that the two methods yield equivalent results when applied to the same data.
When exposure and disease occurrence are quantitatively related, categorisation of a continuous exposure variable may produce differential misclassification and bias estimates of association in a positive or negative direction.^{8,}^{19–}^{21} In an earlier paper, we illustrated the operation of this bias in exposureresponse analyses in which categories of exposure are represented by assigned scores.^{10} Exposure scores derived from the category midpoints produce negative bias, while scores based on category means, as in the examples in this paper, bias associations in a positive direction. The bias is likely to be small if exposure categories are narrowly defined and scores are assigned based on persontime weighted mean values.^{10} Nonetheless, concerns about the consequences of exposure categorisation and score assignment arise frequently in quantitative risk assessment; the literature includes many examples of researchers evaluating the sensitivity of study results to categorisation by varying boundaries and rules about score assignment.^{22–}^{26} Such approaches are time consuming and ultimately never conclusive. An approach that does not require categorisation would clearly be useful.
The approach to Poisson regression analysis of ungrouped persontime data we describe can be used to test the sensitivity of the results to the investigator’s decisions about exposure categorisation and score assignment. There are distinct advantages in retaining the Poisson regression approach for this purpose. An alternative would be to use a different method, such as Cox proportional hazards or conditional logistic regression, to evaluate questions about the impact on risk estimates of categorising exposure data. However, this would entail a different regression model with different assumptions. Furthermore, in our experience, while contemporary computers have substantially reduced the obstacles to fitting Cox regression models for analyses of data from studies of large occupational cohorts, computational obstacles remain when attempting to fit models that involve interactions between a time dependent variable and the timescale specified for the Cox model (for example, an interaction between a baseline timescale of attained age and a time dependent indicator of active employment status). Again, in such cases, the approach to Poisson regression analysis of ungrouped persontime data offers a simple, direct way to evaluate the sensitivity of the results to decisions about exposure categorisation and score assignment.
The ungrouped method that we illustrate sacrifices much of the computational efficiency obtained by grouped data analyses of cohort data. Poisson regression analyses in which ungrouped data are generated with a unique observation for each personday at risk are typically the most refined classification of study data. In many cases, computational efficiency can be gained by a less refined categorisation of persontime (for example, personyears). As illustrated in this paper, however, given the advances in the processing speed of personal computers it is no longer necessary to limit analyses to grouped data approaches to Poisson regression.
Acknowledgments
We thank John Bailer, David Kriebel, Steve Marshall, and Bob Park for constructive comments on earlier versions of this paper.
REFERENCES
Footnotes

Competing interests: none