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Chromium (VI) induced oxidative damage to DNA: increase of urinary 8-hydroxydeoxyguanosine concentrations (8-OHdG) among electroplating workers
  1. H W Kuo,
  2. S F Chang,
  3. K Y Wu,
  4. F Y Wu
  1. Institute of Environmental Health, China Medical College, Taiwan
  1. Correspondence to:
 Dr H W Kuo, Institute of Environmental Health, China Medical College, 91 Hsueh-Shin Road, Taichung, Taiwan, ROC; 
 wukuo{at}mail.cmc.edu.tw

Abstract

Aims: To investigate the concentration of urinary 8-hydroxydeoxyguanosine (8-OHdG) among electroplating workers in Taiwan.

Methods: Fifty workers were selected from five chromium (Cr) electroplating plants in central Taiwan. The 20 control subjects were office workers with no previous exposure to Cr. Urinary 8-OHdG concentrations were determined using high performance liquid chromatography with electrochemical detection.

Results: Urinary 8-OHdG concentrations among Cr workers (1149.5 pmol/kg/day) were higher than those in the control group (730.2 pmol/kg/day). There was a positive correlation between urinary 8-OHdG concentrations and urinary Cr concentration (r = 0.447, p < 0.01), and urinary 8-OHdG correlated positively with airborne Cr concentration (r = 0.285). Using multiple regression analysis, the factors that affected urinary 8-OHdG concentrations were alcohol, the common cold, and high urinary Cr concentration. There was a high correlation of urinary 8-OHdG with both smoking and drinking, but multiple regression analysis showed that smoking was not a significant factor. Age and gender were also non-significant factors.

Conclusion: 8-OHdG, which is an indicator of oxidative DNA damage, was a sensitive biomarker for Cr exposure.

  • chromium (VI)
  • oxidative stress
  • 8-hydroxydeoxyguanosine
  • urine
  • electroplating workers
  • 8-OHdG, 8-hydroxydeoxyguanosine
  • AAS, atomic absorption spectrophotometry
  • BMI, body mass index
  • Cr, chromium
  • CV, coefficient of variation
  • ECD, electrochemical detection
  • HPLC, high pressure liquid chromatography
  • ROS, reactive oxygen species
  • SPE, solid phase extraction
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