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An improved method of analysing 2,5-hexanedione in urine.
  1. L Perbellini,
  2. D M Marhuenda Amoros,
  3. A C Cardona Llorens,
  4. C Giuliari,
  5. F Brugnone
  1. Institute of Occupation Medicine, University of Verona, Policlinico Borgo Roma, Italy.


    A short gas-chromatographic method for analysing urinary concentrations of 2,5-hexanedione is based on acid hydrolysis of urine at pH below 0.1 and "purification" of the urine samples by microcolumns containing an octadecyl-silane phase. A 5% acetonitrile solution allows a fairly selective elution of 2,5-hexanedione from the microcolumns. Recovery of 2,5-hexanedione from urine is as great as 79.9%. The variation coefficient of the measurements is 2.8%. The results obtained from different working conditions and using packed or wide bore or capillary gas-chromatographic columns are reported.

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