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A simplified method for rapid determination of porphyrins by thin-layer chromatography.
  1. T Okuda,
  2. H Nakajima,
  3. K Yatsuki,
  4. M Amano,
  5. G Umeda


    Free porphyrins, especially copro-, uro-, and protoporphyrin, were successfully separated by talc thin-layer chromatography (TLC), and were determined from the relationship of the weight and the area of porphyrins in the spot on the thin-layer plate. The results obtained from both the solvent extraction and the simple, rapid TLC method reported in this paper showed good correlation for coproporphyrin levels up to 400 microgram/1. The TLC method was applied to the determination of urinary coproporphyrin in lead workers and in rats.

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