The prevalence of hepatitis B virus (HBV) markers in 416 dental students, 115 dentists, and 329 members of the general population was studied in Athens. Markers were present in 36 students (8.7%), 34 dentists (29.6%), and 74 controls (22.5%). Four students (1.0%), 3 dentists (2.6%), and 6 controls (1.8%) were carriers of hepatitis B surface antigen (HBsAg). The prevalence of HBV infections increased with age significantly faster in the dental profession than in the general population, showing an increased risk. Of 22 dentists in practice for more than 20 years, 12 (54.5%) had HBV markers and 4 (18.2%) reported a clinical hepatitis infection. These data show that members of the dental profession should also be included in the national hepatitis B vaccination program.