To provide information concerning potential occupational transmission of hepatitis A virus (HAV) among wastewater workers in a large city in the United States, a cross-sectional survey was performed using a saliva test to detect antibodies to HAV (anti-HAV). Fifty-nine (20%) of 302 participants tested positive for anti-HAV. After controlling for the confounding effects of age and race, wastewater work was not significantly associated with an increase in the prevalence of anti-HAV (prevalence ratio = 1.3; 95% confidence interval 0.7 to 2.4). Additionally, when examining only the wastewater workers, no statistically significant occupational risk factors for anti-HAV were identified. The results of this survey are consistent with the position of the Centers for Disease Control and Prevention regarding groups at risk for HAV infection.