Special contributionEndocrine screening methods workshop report: Detection of estrogenic and androgenic hormonal and antihormonal activity for chemicals that act via receptor or steroidogenic enzyme mechanisms☆
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2019, ChemosphereCitation Excerpt :The differences between yeast-based and mammalian in vitro assays regarding ligand potencies have been already reported in the literature. Impermeability of the yeast cells, the absence of cell-specific factors, yeast-strain-specific differences in metabolism, modulation of bioavailability by protein binding are among the most frequently discussed (Lyttle et al., 1992; Arnold et al., 1996; Gray et al., 1997; et al., 1999; Baker, 2001). Sensitivity of the yeast assays is generally an order of magnitude lower in comparison with mammalian in vitro assays (Zacharewski, 1997; Legler et al., 2002).
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2018, Current Opinion in ToxicologyCitation Excerpt :For several decades, it has been known that exogenous chemicals can interact with the endocrine system, possibly leading to adverse effects in humans and ecologically-relevant species [1,2].
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2018, Comprehensive Toxicology: Third EditionDeoxynivalenol induces toxic effects in the ovaries of pigs: An ex vivo approach
2017, TheriogenologyCitation Excerpt :Data about the toxicity of DON on reproductive system have used mainly cell line models focusing changes on oocytes or granulosa cell maturation [17,21]. In addition to cell line models, ovary explants culture has been used as an in vitro system for identifying endocrine-active compounds that alter steroid biosynthesis [22]. The main advantage of the explant model is the possibility to evaluate tissue morphology, maintaining the complex patterns of differentiation seen in vivo and bringing different results from that obtained with isolated single cell types.
Effects of model aromatizable (17α-methyltestosterone) and non-aromatizable (5α-dihydrotestosterone) androgens on the adult mummichog (Fundulus heteroclitus) in a short-term reproductive endocrine bioassay
2015, Comparative Biochemistry and Physiology Part - C: Toxicology and PharmacologyCitation Excerpt :The presence of androgens in pulp mill effluent (PME) may be due to bacterial metabolism of plant sterols and other steroids commonly found in PME (Jenkins et al., 2001; Parks et al., 2001; Carson et al., 2008). Anti-androgenic responses to sewage can occur due to the ability of presumed estrogenic substances to also act as androgen receptor (AR) antagonists to disrupt endocrine function (Gray et al., 1997; Batty and Lim, 1999). A two-year study conducted on waste from cattle feeding pens found that more than 90% of samples collected after a heavy rain contained androsterone and 4-androstenedione, potent androgenic substances (Bartelt-Hunt et al., 2012).
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The research described in this article has been reviewed by the National Health and Environmental Effects Research Laboratory, U.S. Environmental Protection Agency, and approved for publication. Approval does not signify that the contents necessarily reflect the views and policies of the Agency nor does mention of trade names or commercial products constitute endorsement or recommendation for use.