Predominance of type 2 cytokine–producing CD4+ and CD8+ cells in patients with atopic dermatitis☆,☆☆,★,★★
Section snippets
Subjects
Peripheral blood was obtained from 45 patients with AD (19 men and 26 women, aged 25.4 ± 8.6 years [mean ± SD]). AD was diagnosed according to the criteria of Hanifin and Rajka.28 The severity of disease was determined by the criteria of Rajka and Langeland,29 and patients were categorized into three groups. Briefly, symptoms of AD were scored (1 to 3 points in each parameter) by using the following parameters: (1) extent, (2) course (by history), and (3) intensity (disturbance of night's sleep
Frequency of CD4+ and CD8+ cells from patients with AD and from healthy control subjects
To assess the effect of treatment with fixation and permeabilization on staining of surface CD4 and CD8 molecules, we examined frequencies of CD4+ cells and CD8+ cells in treated and untreated samples. Isolated PBMCs were stained with anti-cytokine mAbs, followed by staining of surface CD4 and CD8 antigens with or without fixation in 2% paraformaldehyde and with or without permeabilization in 0.1% saponin. There was no difference in the frequency of CD4+ or CD8+ cells between treated samples
Discussion
In this article, we have demonstrated that the frequency of IL-4–producing CD4+ cells in PBMCs from patients with AD was significantly higher than that from healthy control subjects. In contrast, the frequency of IFN-γ–producing CD4+ cells from patients with AD was significantly lower than that from healthy control subjects when PBMCs were stimulated with immobilized anti-CD3 mAb for 6 hours. Also, among CD8+ cells, the frequency of IL-4–producing cells from patients with AD was significantly
References (37)
- et al.
Heterogeneity of cytokine secretion patterns and functions of helper T cells
Adv Immunol
(1989) - et al.
Specific IgE antibodies in atopic eczema
J Allergy Clin Immunol
(1975) - et al.
Age-related serum immunoglobulin E levels in healthy subjects and in patients with allergic disease
J Allergy Clin Immunol
(1980) Regulation of IgE synthesis in humans
J Allergy Clin Immunol
(1992)Novel fundamental approaches to intervening in IgE-mediated allergic disease
J Invest Dermatol
(1994)- et al.
Shifts in interleukin-4 and interferon-γ production by T cells of patients with elevated serum IgE levels and the modulatory effects of these lymphokines on spontaneous IgE synthesis
J Allergy Clin Immunol
(1991) - et al.
Decreased interferon gamma and increased interleukin-4 production in atopic dermatitis promotes IgE synthesis
J Allergy Clin Immunol
(1992) - et al.
Enhanced IL-4 production and IL-4 receptor expression in atopic dermatitis and their modulation by interferon-gamma
J Invest Dermatol
(1992) - et al.
Detection of intracellular cytokines by flow cytometry
J Immunol Methods
(1993) - et al.
Decreased frequency of interferon-γ- and interleukin-2-producing cells in patients with atopic diseases measured at the single cell level
J Allergy Clin Immunol
(1995)
Enumeration of IFN-γ-producing cells by flow cytometry: comparison with fluorescence microscopy
J Immunol Methods
Cytokine-induced differentiation of precursor mouse CD8+ T cells into cytotoxic CD8+ T cells secreting Th1 or Th2 cytokines
Immunity
Two types of murine helper T cell clones. I. Definition according to profiles of lymphokine activities and secreted proteins
J Immunol
Purified protein derivative of Mycobacterium tuberculosis and excretory-secretory antigen(s) of Toxocara canis expand in vitro human T cells with stable and opposite (type 1 T helper or type 2 T helper) profile of cytokine production
J Clin Invest
Selection of a human T helper type 1-like T cell subset by mycobacteria
J Exp Med
Alloreactive murine CD8+ T cell clones secrete the Th1 pattern of cytokines
J Immunol
Alloreactive cloned T cell lines. VI
Multiple lymphokine activities secreted by helper and cytolytic cloned T lymphocytes. J Immunol
Simultaneous production of IL-2, IL-4, and IFN-γ by activated human CD4+ and CD8+ T cell clones
J Immunol
Cited by (0)
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From athe Department of Immunology and Parasitology, bthe Department of Ophthalmology, and cthe Department of Dermatology, School of Medicine, Yokohama City University, Yokohama.
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Supported in part by Grants-in-Aid from the Japanese Ministry of Education, Science and Culture and by grants from Yokohama City.
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Reprint requests: Mutsuhiko Minami MD, PhD, Department of Immunology and Parasitology, School of Medicine, Yokohama City University, 3-9 Fukuura, Kanazawaku, Yokohama, 236 Japan.
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