Elsevier

Clinica Chimica Acta

Volume 73, Issue 3, December 1976, Pages 453-461
Clinica Chimica Acta

Rapid colorimetric assay of β-galactosidase and N-acetyl-β-glucosaminidase in human urine

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Abstract

Colorimetric methods using 4-nitrophenyl-glycoside substrates for the assay of β-galactosidase and N-acetyl-β-glucosaminidase in human urine are described. Interfering substances were removed by gel-filtration of urine. An unidentified low-molecular-weight inhibitor of N-acetyl-β-glucosaminidase was found. Increased sensitivity of the methods was achieved by high sample volumes, small volumes of buffer to terminate the enzyme reaction and optimal substrate concentration and buffer pH. Incubation periods were shortened to 15 min. Both methods were designed as single-tube tests in which buffer-substrate solutions are prepared in bulk and aliquote are stored in individual containers at-25° C. Under these conditions reagents were stable for at least six months. Precision of both methods was satisfactory. Estimates of normal limits are reported.

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