Elsevier

Clinica Chimica Acta

Volume 43, Issue 3, 12 February 1973, Pages 305-310
Clinica Chimica Acta

Determination of serum creatinine by a direct colorimetric method

https://doi.org/10.1016/0009-8981(73)90466-XGet rights and content

Abstract

The Jaffe reaction has been modified by the addition of sodium-dodecyl-sulfate (SDS) and borate to the reaction mixture. A method has been elaborated permitting serum creatinine determination without prior deproteinisation.

On direct serum creatinine determination using this reagent, values are obtained that correlate well to those obtained with a method involving isolation of creatinine with a chromatographic procedure.

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Present address: Department of Physiological Chemistry II, Chemical Center, Box 740, Lund 7, Sweden.

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