Skip to main content
SpringerLink
Log in

Endotoxin inhalation induces neutrophil chemotaxis by alveolar macrophages

  • Immunosuppression and Inflammation
  • Published:
Agents and Actions Aims and scope Submit manuscript

Abstract

Guinea-pigs were exposed to an aerosol of bacterial endotoxin (lipopolysaccharide, LPS). The free lung cell response and alveolar macrophage (AM) chemotaxis were studied. Neutrophils from guinea-pig blood gave larger migration responses than those obtained by intraperitoneal glycogen stimulation or human neutrophils. An increase in the number of neutrophils in the airways was found with a peak at 12–24 hours after exposure. In animals pre-treated with LPS inhalation for 4 months, the reaction was of shorter duration and smaller magnitude. AM showedin vitro chemotaxtic activity up to 4 hours after exposure; no difference was found in pre-treated animals. The results suggest that the neutrophil invasion in the airways after LPS is depedent on two mechanisms, the initial being AM chemotaxis, which is not modified by pre-exposure to LPS, and another unknown factor, which is modified by pre-exposure to LPS.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. S.K. Ainsworth andR.E. Neuman,Chemotaxins in cotton mill dust: possible etiologic agent(s) in byssinosis, Am. Rev. resp. Dis.124, 280–284 (1981).

    PubMed  Google Scholar 

  2. C.A. Bona,Fate of endotoxin in macrophages: biological and ultrastructural aspects, J. infect. Dis.128, 66–73 (1973).

    Google Scholar 

  3. S.K. Bult andA.G. Herman,Thromboxane A 2.Biosynthesis during endotoxin induced aggregation of platelets from normal and sensitized guinea pigs, Agents and Actions9, 560–565 (1979).

    Article  PubMed  Google Scholar 

  4. F. Dallegri, G. Lanzi andF. Patrone.Evidence for a reversible functional state of neutrophil chemotactic deactivation, Int. Archs Allergy appl Immun.63, 330–337 (1980).

    Google Scholar 

  5. J.E. Gadek, G.W. Hunninghake, R.L. Zimmerman andR.G. Crystal,Regulation of the release of alveolar macrophage derived neutrophil chemotactic factor, Am. Rev. resp. Dis.121, 723–733 (1980).

    PubMed  Google Scholar 

  6. D.J. Gorenberg andR.P. Daniele,Characterization of immunocompetent cells recovered from the respiratory tract and tracheobronchial lymph node of normal guinea-pigs, Am. Rev. resp. Dis.114, 1099–1105 (1976).

    PubMed  Google Scholar 

  7. I. Helander,Acute pulmonary toxicity and pyrogenicity of inhaled Lipid A, FEMS Microbiol. Lett.13, 283–287 (1982).

    Article  Google Scholar 

  8. I. Helander, J. Piiparinen andR. Rylander,Acute pulmonary toxicity of lipopolysaccharides in defined salt forms, FEMS Microbiol. Lett.15, 123–127 (1982).

    Article  Google Scholar 

  9. R.M. Kaelin, D.M. Center, J. Bernardo, M. Grant andG.L. Snider,The role of macrophagederived chemoattranctant activities in the early inflammatory events of bleomycin-induced pulmonary injury, Am. Rev. resp. Dis.128, 132–137 (1983).

    PubMed  Google Scholar 

  10. H.U. Keller, H. Gerber, M.W. Hess andH. Cottier,Studies on the regulation of the neutrophil chemotactic response using a rapid and reliable method for measuring random migration and chemotaxis of neutrophil granulocytes Agents and Actions6, 326–339 (1976).

    Article  PubMed  Google Scholar 

  11. M.S. Klempner andR.E. Rocklin,Effects of leukocyte inhibitory factor (LIF) on human neutrophil function, Inflammation7, 145–153 (1983).

    Article  PubMed  Google Scholar 

  12. E.M. Lugano, J.H. Dauber andR.P. Daniele,Acute experimental silicosis. Lung morphology, history and macrophage chemitaxin secretion, Am. J. Path.109, 27–36 (1982).

    PubMed  Google Scholar 

  13. R. Rylander,Free lung cells studies cigarette smoke inhalation experiments, Scand. J. Resp. Dis.52, 121–128 (1971).

    Google Scholar 

  14. R. Rylander andJ. Vesterlund, Airborne endotoxins in various occupational environments. InEndotoxins and Their Detection with the Limulus Amebocyte Lysate Test, pp. 399–408 (EdsS.W. Watson, J. Levin andT.J. Novitsky) Alan R. Liss Inc, New York 1982.

    Google Scholar 

  15. J.P. Schreider andJ.O. Hutchens,Particles deposition in the guinea-pig respiratory tract, J. Aerosol. Sci.10, 599–607 (1979).

    Article  Google Scholar 

  16. M.-C. Snella andR. Rylander,Lung cell reactions after inhalation of bacterial lipopolysaccharides, Eur. J. resp. Dis.63, 550–557 (1982).

    Google Scholar 

  17. R.I. Walker andW.J. Beasley,Evidence that antipolysaccharide antibodies alter platelet response to endotoxin in tolerant rabbits, Can. J. Microbiol.26, 1241–1246 (1980).

    PubMed  Google Scholar 

  18. O. Yamada, C.F. Moldow, T.M. Sacks, P.R. Craddock, M.A. Boogaerts andH.S. Jacob,Deleterious effects of endotoxin on cultured endothelial cells an in vitromodel for vascular injury, Inflammation5, 115–126 (1981).

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Social and Preventive Medicine, University of Geneva, 27, Quai Charles Page, CH-1211, Geneva 4, Switzerland

    Marie-Claire Snella

  2. Department of Environmental Hygiene, University of Gothenburg, Gothenburg, Sweden

    Ragnar Rylander

Authors
  1. Marie-Claire Snella
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ragnar Rylander
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Snella, MC., Rylander, R. Endotoxin inhalation induces neutrophil chemotaxis by alveolar macrophages. Agents and Actions 16, 521–526 (1985). https://doi.org/10.1007/BF01983657

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01983657

Keywords

Search

Navigation