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Seroprevalence of lyme borreliosis in forestry workers from Brandenburg, Germany

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Abstract

In 1992 blood samples were taken from 630 forestry workers in the state of Brandenburg, Germany, and an inquiry about tick bites and possible symptoms of Lyme borreliosis carried out in order to determine the seroprevalence of the disease. To estimate the rate of seroconversion within six months, 406 of the individuals were investigated a second time. IgG and IgM antibodies againstBorrelia burgdorferi were detected in serum using an indirect immunofluorescence assay (IFA) and an immunoblot assay (IBA). Fifty-three percent of the forestry workers reported suffering a tick bite, 8% of whom recalled an erythema after the bite. Positive results were found more frequently in the forestry workers than in a control group of 200 healthy blood donors in both the IgG-IFA (8% vs. 4%, p<0.05) and the IgG-IBA (18% vs. 5%, p<0.05). The detection of IgG antibodies correlated with a tick bite and erythema history. There was a tendency of lower seropositivity by the IgG-IBA in individuals who treated the ticks before removal with chemicals or other agents compared to those without such treatment (16.8% vs. 23.9%, 0.05 >p<0.1). Likewise, there was a tendency of lower seropositivity by the IgG-IFA in individuals being treated with antibiotics for other reasons compared to untreated individuals (3.15% vs. 8.9%, p<0.05), although the two groups did not differ in the IgG-IBA (13.8% vs. 18.5%, p>0.1). The rate of seroconversion within six months ranged from 5 to 7%. It is concluded that forestry workers in Brandenburg, Germany, are at risk for infection withBorrelia burgdorferi, but clinical signs of infection are rare.

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References

  1. Jaenson TGT: The epidemiology of Lyme borreliosis. Parasitology Today 1991, 7: 39–45.

    PubMed  Google Scholar 

  2. Steere AC: Lyme disease. New England Journal of Medicine 1989, 321: 586–596.

    PubMed  Google Scholar 

  3. Berglund J, Eitrem R: Tick-borne borreliosis in the archipelage of southern Sweden. Scandinavian Journal of Infectious Diseases 1993, 25: 67–72.

    PubMed  Google Scholar 

  4. Blaauw I, Nohlmans L, van den Bogaard T, van der Linden S: Diagnostic tools in Lyme borreliosis: clinical history compared with serology. Journal of Clinical Epidemiology 1992, 45: 1229–1236.

    PubMed  Google Scholar 

  5. Cinco M, Balanzin D, Benussi R Trevisan G: Seroprevalence and incidence of Lyme Borreliose in forestry workers in Friuli Venezia Giulia (Northern Italy). Alpe Andria Microbiology Journal 1993, 2: 91–98.

    Google Scholar 

  6. Fahrer H, Sauvain MJ, van der Linden S, Zhioua E, Gern L, Aeschlimann A: Prävalenz der Lyme-borreliosis in einer schweizerischen Risikopopulation. Schweizerische Medizinische Wochenschrift 1988, 118: 65–69.

    PubMed  Google Scholar 

  7. Gern L, Frossard E, Walter A, Aeschlimann A: Presence of antibodies againstBorrelia burgdorferi in a population of the Swiss plateau. Zentralblatt für Bakteriologie 1989, Supplement 18: 321–328.

    Google Scholar 

  8. Goldstein MD, Schwartz BS, Friedmann C, Maccarillo B, Borbi M, Tuccillo R: Lyme disease in New Jersey outdoor workers: a statewide survey of seroprevalence and tick exposure. American Journal of Public Health 1990, 80: 1225–1229.

    PubMed  Google Scholar 

  9. Gustafson R, Svenungsson B, Gardulf A, Stiernstedt G, Forsgren M: Prevalence of tick-borne encephalitis and Lyme borreliosis in a defined Swedish population. Scandinavian Journal of Infectious Diseases 1990, 22: 297–306.

    PubMed  Google Scholar 

  10. Gustafson R, Forsgren M, Gardulf A, Grandström M, Svenungsson B: Antibody prevalence and clinical manifestations of Lyme borreliosis and tick-borne encephalitis in Swedish orienteers. Scandinavian Journal of Infectious Diseases 1993, 25: 605–611.

    PubMed  Google Scholar 

  11. Guy EC, Bateman DE, Martyn CN, Heckels JE, Lawton NF: Lyme disease: prevalence and clinical importance ofBorrelia burgdorferi specific IgG in forestry workers. Lancet 1989, i: 484–486.

    Google Scholar 

  12. Hassler D, Zöller L, Haude M, Hufnagel HD, Sonntag HG: Lyme-Borreliose in einem europäischen Endemiegebiet. Deutsche Medizinische Wochenschrift 1992, 117: 767–774.

    PubMed  Google Scholar 

  13. Huycke MM, D'Alessio DD, Marx JJ: Prevalence of antibodies toBorrelia burgdorferi by indirect fluorescent antibody assay, ELISA, and Western immunoblot in healthy adults in Wisconsin and Arizona. Journal of Infectious Diseases 1992, 165: 1133–1137.

    PubMed  Google Scholar 

  14. Kuiper H, de Jongh BM, Nauta AP, Houweling H, Wiessing LG, Moll van Charante AW, Spanjaard L: Lyme borreliosis in Dutch forestry workers. Journal of Infection 1991, 23: 279–286.

    PubMed  Google Scholar 

  15. Kuiper H, van Dam AP, Moll van Charante AW, Nauta NP, Dankert J: One year follow-up study to assess the prevalence and incidence of Lyme borreliosis among Dutch forestry workers. European Journal of Clinical Microbiology & Infectious Diseases 1993, 12: 413–418.

    Google Scholar 

  16. Lane RS, Manweiler SA, Stubbs HA, Lennette ET, Madigan JE, Lavoie PE: Risk factors for Lyme disease in a small rural community in northern California. American Journal of Epidemiology 1992, 136: 1358–1368.

    PubMed  Google Scholar 

  17. Münchhoff P, Wilske B, Preac-Mursic V, Schierz G: Antibodies againstBorrelia burgdorferi in Bavarian forest workers. Zentralblatt für Bakteriologie Reihe A 1986, 263: 412–419.

    Google Scholar 

  18. Nadal D, Wunderli W, Briner H, Hansen K: Prevalence of antibodies toBorrelia burgdorferi in forestry workers and blood donors from the same region in Switzerland. European Journal of Clinical Microbiology & Infectious Diseases 1989, 8: 992–995.

    Google Scholar 

  19. Neubert U, Münchhoff P, Völker B, Reimers CD, Pflüger KH:Borrelia burgdorferi infections in Bavarian forest workers. Annals of the New York Academy of Sciences 1988, 539: 476–479.

    Google Scholar 

  20. Pejcoch M, Kralikova Z, Strnad P, Stanek G: Prevalence of antibodies toBorrelia burgdorferi in forestry workers of south Moravia. Zentralblatt für Bakteriologie 1988, Supplement 18: 317–320.

    Google Scholar 

  21. Pierer K, Köck T, Freidl W, Stünzner D, Pierer G, Marth E, Lechner H, Möse JR: Prevalence of antibodies toBorrelia burgdorferi flagellin in Syrian blood donors. Zentralblatt für Bakteriologie 1993, 279: 239–243.

    Google Scholar 

  22. Rees DHE, Axford JS: Evidence for Lyme disease in urban park workers: a potential new health hazard for city inhabitants. British Journal of Rheumatology 1994, 33: 123–128.

    PubMed  Google Scholar 

  23. Satz N, Ackermann R, Gern L, Aeschlimann A, Ott A, Knoblauch M: Zur Epidemiologie der Infektion mitBorrelia burgdorferi. Schweizerische Medizinische Wochenschrift 1988, 118: 422–426.

    PubMed  Google Scholar 

  24. Schmidt R, Gollmer E, Zunser R, Krüger J, Ackermann R: Prevalence of erythema migrans borreliosis in blood donors. Infusionstherapie 1989, 16: 248–251.

    PubMed  Google Scholar 

  25. Schwartz BS, Goldstein MD: Lyme disease in outdoor workers: risk factors, preventive measures, and tick removal methods. American Journal of Epidemiology 1990, 131: 877–885.

    PubMed  Google Scholar 

  26. Smith PF, Benach JL, White DJ, Stroup DF, Morse DL: Occupational risk of Lyme disease in endemic areas of New York State. Annals of the New York Academy of Sciences 1988, 539: 289–301.

    PubMed  Google Scholar 

  27. Sticht-Groh V, Martin R, Schmidt-Wolf I: Antibody titer determinations againstBorrelia burgdorferi in blood donors and two different groups of patients. Annals of the New York Academy of Sciences 1988, 539: 497–499.

    Google Scholar 

  28. Wilske B, Münchhoff P, Schierz G, Preac-Mursic V, Roggendorf M, Zoulek G: Zur Epidemiologie derBorrelia burgdorferi-Infektion. Münchner Medizinische Wochenschrift 1985, 127: 171–172.

    Google Scholar 

  29. Bakken LL, Case KL, Callister SM, Bourdeau NJ, Schell RF: Performance of 45 laboratories participating in a proficiency testing program for Lyme disease serology. Journal of the American Medical Association 1992, 268: 891–895.

    PubMed  Google Scholar 

  30. Zöller L, Burkard S, Schäfer H: Validity of Western immunoblot band pattern in the serodiagnosis of Lyme borreliosis. Journal of Clinical Microbiology 1991, 29: 174–182.

    PubMed  Google Scholar 

  31. Ma B, Christen B, Leung D, Vigo-Pelfrey C: Serodiagnosis of Lyme borreliosis by Western immunoblot: reactivity of various significant antibodies againstBorrelia burgdorferi. Journal of Clinical Microbiology 1992, 30: 370–376.

    PubMed  Google Scholar 

  32. Dressler F, Whalen JA, Reinhardt BN, Steere AC: Western blotting in the serodiagnosis of Lyme disease. Journal of Infectious Diseases 1993, 167: 392–400.

    PubMed  Google Scholar 

  33. Rath P-M, Marsch W, Brade V, Fehrenbach F-J: Serological distinction between syphilis and Lyme borreliosis. Zentralblatt für Bakteriologie 1994, 280: 319–324.

    Google Scholar 

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Author notes

  1. P. M. Rath

    Present address: Institut für Medizinische Mikrobiologie, Universität - GH Essen, Hufelandstrasse 55, 45147, Essen, Germany

Authors and Affiliations

  1. Abteilung für Mikrobiologie, Universitätsklinikum Rudolf Virchow, Freie Universität Berlin, Augustenburger Platz 1, 13344, Berlin, Germany

    P. M. Rath

  2. Robert Koch-Institut, Nordufer 20, 13352, Berlin, Germany

    B. Ibershoff, J. Albig, B. Eljaschewitsch, D. Jürgens, I. Horbach & F. J. Fehrenbach

  3. Forschungs-EDV, Universitätsklinikum Rudolf Virchow, Spandauer Damm 130, 14050, Berlin, Germany

    A. Mohnhaupt

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Rath, P.M., Ibershoff, B., Mohnhaupt, A. et al. Seroprevalence of lyme borreliosis in forestry workers from Brandenburg, Germany. Eur. J. Clin. Microbiol. Infect. Dis. 15, 372–377 (1996). https://doi.org/10.1007/BF01690092

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