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An assessment of a urinary biomarker for total human environmental exposure to benzo[a]pyrene

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Abstract

Urinary banzo[a]pyrene (BaP) metabolite levels were compared to human environmental exposure to BaP through inhalation and dietary ingestion to assess the predictive validity of the exposure biomarker. These measurements were made for 14 adult volunteers over 14 consecutive days, once during summer/fall, again during winter periods. Based on personal air monitoring, median potential inhalation doses of 11.0 and 2.3 ng/day were estimated for the winter and summer/fall studies, respectively. A median potential ingested dose of 176 ng/day, estimated from “duplicate plate” sampling, exceeded inhalation by 6-and 122-fold for the winter and summer/fall studies, respectively. “Total” urinary BaP metabolites were measured using a published “reverse metabolism” (BaP) method of analysis. Median rates of urinary BaP metabolite elimination for the winter and summer/fall studies were 121 and 129 ng/day, respectively. The changes in inhaled and ingested potential doses were regressed on the change in urinary metabolite elimination from week 1 to week 2 to test the predictive validity of the biomarker measurement. The regression was statistically significant (r = 0.620, p = 0.015, n = 25) when body weight was included and two extreme values were removed. Consistent with the exposure measurements showing diet as the dominant route of exposure, most of the variation in urinary metabolite elimination was explained by the ingested dose. It is concluded that the measurement of urinary BaP by “reverse metabolism” is qualitative and of marginal predictive validity as an exposure biomarker due to the method's low recoveries and the large unexplained variance.

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References

  • Alessio L, Berlin A, Dell'Orto A, Toffoletto F, Ghezzi I (1985) Reliability of urinary creatinine as a parameter used to adjust values of urinary biological indicators. Int Arch Occup Environ Health 55:99–106

    Google Scholar 

  • Becher G, Bjorseth A (1983) Determination of exposure to polycyclic aromatic hydrocarbons by analysis of human urine. Cancer Lett 17:301–311

    Google Scholar 

  • Becher G, Bjorseth A (1984) Multimethod determination of occupational exposure to polycyclic aromatic hydrocarbons in an aluminum plant. Carcinogenesis 5:647–651

    Google Scholar 

  • Buckley TJ (1991) Benzo[a]pyrene (BaP) metabolites and 1-hydroxypyrene as urinary biomarkers for human environmental exposure to BaP. Department of Environmental Science, Rutgers: The State University of New Jersey, Piscataway, NJ (dissertation)

    Google Scholar 

  • Buckley TJ, Lioy PJ (1992) An examination of the time course from human dietary PAH exposure to urinary elimination of 1-hydroxypyrene. Br J Ind Med 49:113–124

    Google Scholar 

  • Buckley TJ, Waldman JM, Lioy PJ (1988) High-flow, 24-hour personal sampling: Problems and solutions. Paper No. 88-115.5, Proceedings of the 81st Annual Meeting of the Air Pollution Control Association, Pittsburgh, Pa.

  • Buckley TJ, Waldman JM, Freeman NCG, Lioy PJ (1991) Calibration intersampler comparison and field application of a new PM-10 personal air sampling impactor. Aerosol Sci Technol 14:380–387

    Google Scholar 

  • Butler JP, Post GB, Lioy PJ, Waldman JM, Greenberg A (1993) Assessment of carcinogenic risk from personal exposure to benzo[a]pyrene in the total human environmental exposure study (THEES). J Air Waste Management Assoc 43:970–976

    Google Scholar 

  • Chuang JC, Callahan PJ, Gordon SM (1993) Methods for polycyclic aromatic hydrocarbons and tobacco smoke markers in house dust. Proceedings of the 1993 EPA/AWMA International Symposium Measurement of Toxic and Related Air Pollutants. Pittsburgh, Pa., pp 88–93

  • Dennis JM, Massey RC, McWeeny DJ, Watson DH (1983) Polycyclic aromatic hydrocarbons in the U.K. diet. Fd Chem Toxicol 5:569–574

    Google Scholar 

  • De Vos RH, Van Dokkum W, Schouten A, Jong-Berkhout P (1990) Polycyclic aromatic hydrocarbons in Dutch total diet samples. Fd Chem Toxicol 28:263–268

    Google Scholar 

  • Diem K, Lentner C (1970) Documenta Geigy, CIBA-GEIGY, Basle, Switzerland

    Google Scholar 

  • Ehrenberg L (1988) Dose monitoring and cancer risk. In: Bartsch H, Hemminki K, O'Neill IK (eds) Methods for detecting DNA damaging agents in humans: applications in cancer epidemiology and prevention. IARC Scientific Publications, 89, Lyon

    Google Scholar 

  • Federal Register (May 29, 1992) Guidelines for exposure assessment. 57(104):22887–22938

    Google Scholar 

  • Fowle JR (1984) Workshop proceedings: approaches to improving the assessment of human genetic risk-human biomonitoring. Report No. EPA-600/9-84-016. Washington DC, Office of Health and Environmental Assessment, U.S. Environmental Protection Agency, p 40

    Google Scholar 

  • Freeman NCG, Waldman JM, Lioy PJ (1991) Design and evaluation of a location and activity log used for assessing personal exposure to air pollutants. J Exposure Anal Environ Epidemiol 1:327–338

    Google Scholar 

  • Greenberg A, Luo S, Hsu CH, Creighton P, Waldman JM, Lioy PJ (1990) Benzo[a]pyrene in composite meals: results from the Total Human Environmental Exposure Study (THEES). Polycyclic Aromatic Cmpds 1:221–231

    Google Scholar 

  • Grimmer G, Dettbarn G, Naujack KW, Jacob J (1991) Excretion of hydroxy derivatives of polycyclic aromatic hydrocarbons of the masses 178, 202, 228 and 252 in the urine of coke and road workers. Int J Environ Anal Chem 43:177–186

    Google Scholar 

  • Guthrie H (1981) Paper presented at NE-73 Technical Committee Meeting, Amherst, Mass

  • Harkov R, Greenberg A (1985) Benzo[a]pyrene in New Jersey — results from a twenty-seven site study. JAPCA 35:238–243

    Google Scholar 

  • Hattemer-Frey HA, Travis CC (1991) Benzo-a-pyrene: environmental partitioning and human exposure. Toxicol Ind Hlth 7:141–157

    Google Scholar 

  • Haugen A, Becher G, Benestad C, Vahakangas K, Trivers GE, Newman MJ (1986) Determination of polycyclic aromatic hydrocarbons in the urine, benzo[a]pyrene diol epoxide-DNA adducts in lymphocyte DNA, and antibodies to the adducts in sera from coke oven workers exposed to measured amounts of polycyclic aromatic hydrocarbons in the work atmosphere. Cancer Res 46:4178–4183

    Google Scholar 

  • Howard JW, Fazio T (1980) Review of polycyclic aromatic hydrocarbons in foods. J Assoc Offic Anal Chem 63:1077–1104

    Google Scholar 

  • Howard JW, Fazio T, White RH, Klimech BA (1986) Extraction and estimation of polycyclic aromatic hydrocarbons in total diet. J Assoc Offic Anal Chem 51:122–129

    Google Scholar 

  • Hutcheon DE, Kantrowitz, J, Van Gelder RN, Flynn E (1983) Factors affecting plasma benzo[a]pyrene levels in environmental studies. Environ Res 32:104–110

    Google Scholar 

  • International Commission on Radiological Protection (1974) Report of the task group on reference man. Pergamon Press, New York, pp 335–353

    Google Scholar 

  • Jackson S (1966) Creatinine in urine as an index of urinary excretion rate. Health Phys 12:843–850

    Google Scholar 

  • Jongeneelen FJ, Anzion RBM, Scheepers Box RP, Henderson PTH, Nijenhuis EH, Veenstra SJ, Brouns RME, Winkes A (1988) 1-Hydroxypyrene in urine as a biological indicator of exposure to polycyclic aromatic hydrocarbons in several work environments. Ann Occup Hyg 32:35–43

    Google Scholar 

  • Knuiman JT, Hautvast JGAJ, Van Der Heijden L, Geboers J, Joossens JV, Torngvist H, Isaksson B, Pietinen P, Tuomilehto J, Flynn A, Shortt C, Böing H, Yomtov B, Angelico F, Ricci G (1986) A multi-centre study on within person variability in the urinary excretion of sodium potassium calcium mangesium and creatinine in 8 European centres. Hum Nutr Clin Nutr 40:343–348

    Google Scholar 

  • Konieczny M, Harvey RG (1979) Efficient reduction of polycyclic quinones, hydroquinones, and phenols to polycyclic aromatic hydrocarbons with hydriodic acid. J Org Chem 44:4813–4816

    Google Scholar 

  • Lintas C, DeMatthaeis MC (1979) Determination of benzo[a]-pyrene in smoked, cooked and toasted food products Fd Cosmet Tox 17:325–328

    Google Scholar 

  • Lioy PJ (1990)Assessing total human exposure to contaminants, a multidisciplinary approach. Environ Sci Technol 24:938–945

    Google Scholar 

  • Lioy PJ, Waldman JM, Greenberg A, Harkov R, Pietarinen C (1988) The Total Human Environmental Exposure Study (THEES): comparison of the inhalation and food pathways. Arch Environ Health 43:304–312

    Google Scholar 

  • Lioy PJ, Waldman JM, Buckley TJ, Greenberg A, Butler J, Pietarinen C (1990) The personal, indoor and outdoor concentrations of PM-10 measured in an industrial community during the winter. Atmos Environ 24B:57–66

    Google Scholar 

  • Masuda Y, Kuratsune M (1971) PAH in smoked fish, Katsuobushi. Gann 62:27–30

    Google Scholar 

  • Michels S, Einbrodt HJ (1979) Polycyclic aromatic hydrocarbons in human urines collected in a large industrial city — an epidemiological study. Wissenschaft and Umwelt 3:107–111

    Google Scholar 

  • National Research Council (1989) Report of the oversight committee. In: Grossblatt N, Paulson LR, (eds) Biologic markers in reproductive toxicology. National Academy Press, Washington D.C., pp 15–35

    Google Scholar 

  • National Research Council (1991) Human exposure assessment for airborne pollutants-advances and opportunities. National Academy of Sciences, Washington D.C.

    Google Scholar 

  • Needleman HL, Gatsonis CA (1990) Low-level lead exposure and the IQ of children. JAMA 263:673–678

    Google Scholar 

  • Osborne MR, Crosby NT (1987) Occurrence of benzopyrenes in the environment. In: Coombs MM, Ashby J, Newbold RF, Baxter H, Benzopyrenes. Cambridge University Press, Cambridge

    Google Scholar 

  • Ott WR (1990) Total human exposure: basic concepts, EPA field studies, and future research needs. J Air Waste Manage Assoc 40:966–975

    Google Scholar 

  • Ouyang Z (1989) Study of analytical method for benzo[a]pyrene metabolites in human urine. New Jersey Institute of Technology, Newark, N. J. (Masters dissertation)

    Google Scholar 

  • Ouyang Z, Greenberg A, Kwei GY, Kauffman FC, Faria E (1994) A rapid assay for urinary metabolites of benzo[a]pyrene (B[a]P). Polycyclic Aromatic Cmpds 5:259–268

    Google Scholar 

  • Perera F (1986) New approaches in risk assessment for carcinogens. Risk Anal 6:195–201

    Google Scholar 

  • Santodonato J, Howard P, Basu D (1981) Health and ecological assessment of polynuclear aromatic hydrocarbons. J Environ Pathol Toxicol 5:1–364

    Google Scholar 

  • Schulte PA (1989) A conceptual framework for the validation and use of biological markers. Environ Res 48:129–144

    Google Scholar 

  • Smiciklas-Wright H, Guthrie H (1984) Dietary methodologies: their uses, analyses, interpretations, and implications. In: Simko MD, Corwell C, Gilbridge JA (eds) Nutrition assessment. Aspin, Rockville, Md.

    Google Scholar 

  • Swanson DH, Walling JF (1981) Use of ultrasonics in the rapid extraction of Hi-Vol filters for benzo[a]pyrene (BaP) analysis. Chromatography Newsletter 9:25–26

    Google Scholar 

  • Swanson D, Morris C, Hedgecoke JR, Thompson R, Bumgarner JE (1978) A rapid analytical procedure for the analysis of benzo[a]pyrene in environmental samples. Trends Fluorescence 1:22–27

    Google Scholar 

  • Tolos WP, Shaw PB, Lowry LK, MacKenzie BA, Deng J, Markel HL (1990) 1-Pyrenol: a biomarker for occupational exposure to polycyclic aromatic hydrocarbons. Appl Occup Environ Hyg 5:303–309

    Google Scholar 

  • U.S. Environmental Protection Agency (1983) The human food chain as an environmental exposure pathway. EPA 600/x-83001, Environmental Monitoring Systems Laboratory, Las Vegas, Nev.

    Google Scholar 

  • U.S. Environmental Protection Agency (1989) Exposure Factors Handbook. EPA 600-8-89 043. Office of Health and Environmental Assessment, Washington, D.C.

    Google Scholar 

  • Vaessen HAMG, Jekel AA, Wilbers AAMM (1986) Dietary intake of polycyclic aromatic hydrocarbon. PAH-Symposium, Nijmegen, Netherlands.

  • Venier P, Clonfero E, Cottica D, Gava C, Zordan M, Pozzoli L, Levis AG (1985) Mutagenic activity and polycyclic aromatic hydrocarbon levels in urine of workers exposed to coal tar pitch volatiles in an anode plant. Carcinogenesis 6:749–752

    Google Scholar 

  • Waldman JM, Buckley TJ, Greenberg A, Butler J, Pietarinen C, Lioy PJ (1990) Investigations of indoor and outdoor levels of benzo(a)pyrene in a community of older homes. Polycyclic Aromatic Cmpds 1:137–149

    Google Scholar 

  • Waldman JM, Lioy PJ, Greenberg A, Butler J (1991) Analysis of human exposure to benzo[a]pyrene via inhalation and food ingestion in the total human environmental exposure study (THEES). J Exposure Anal Environ Epidemiol 1:193–225

    Google Scholar 

  • Weston A, Santella R, Bowman E (1994) Detection of polycyclic aromatic hydrocarbon metabolites in urine from coal tar treated psoriasis patients and controls. Polycycl Aromatic Cmpds 5:241–247

    Google Scholar 

  • Xia X, Wu M, Rao Z (1985) Determination of benzo[a]pyrene in Beijing roast duck and roast lamb. Shipih Kexue (Beijing) 61:1–3

    Google Scholar 

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Buckley, T.J., Waldman, J.M., Dhara, R. et al. An assessment of a urinary biomarker for total human environmental exposure to benzo[a]pyrene. Int. Arch Occup Environ Heath 67, 257–266 (1995). https://doi.org/10.1007/BF00409408

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