Summary
A method for isolating 1-hydroxypyrene from urine is described. The presence of 1-hydroxypyrene in urine was identified by fluorescence excitation and emission scanning after HPLC-separation. 1-Hydroxypyrene could be detected in the urine of rats following oral administration of as little as 0.5 μg pyrene. The dose-dependence of 1-hydroxypyrene in urine was evident after a wide range of pyrene dosing. After therapeutical coal tar treatment of dermatological patients the enhanced excretion of 1-hydroxypyrene was highly significant. Employees of a creosote impregnating plant showed an excretion pattern of 1-hydroxypyrene which could be related to their work. 1-Hydroxypyrene in urine of non-exposed people was very low, but detectable. It is suggested that the method reported is suitable for the assessment of uptake of man to pyrene, a compound that is commonly present in work environments which are associated with pollution of polycyclic aromatic hydrocarbons.
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References
Blome H (1983) Polyzyklische aromatische Kohlenwasserstoffe (PAH) am Arbeitsplatz. BIAreport 3/83. BIA, Sankt Augustin, FRG
Bos RP, Theuws JLG, Leijdekkers Ch-M, Henderson PTh (1984a) The presence of the mutagenic polycyclic aromatic hydrocarbons benzo(a)pyrene and benz(a)anthracene in creosote Pl. Mutat Res 130:153–158
Bos RP, Hulshof CTJ, Theuws JLG, Henderson PTh (1984b) Genotoxic exposure of workers creosoting wood. Br J Ind Med 41:260–262
Boyland E, Sims P (1964) Metabolism of polycyclic compounds. 23. The metabolism of pyrene in rats and rabbits. Biochem J 90:391–398
Cernikova M, Dubski H, Horacek J (1983) Detection of acridine in human urine after topical coal tar treatment. J Chromatogr 273:202–206
Clonfero E, Venier P, Toffolo D, Busi L, Levis AG (1983) Urine extracts of workers professionally exposed to polycyclic aromatic hydrocarbons (PAH's), but not smokers are not mutagenic. Abstract of poster presented at Int. Sem. on methods of monitoring human exposure to carcinogenic and mutagenic agents. Espoo, Finland
IARC Monographs (1983) Polynuclear aromatic compounds. Part 1. Chemical, environmental and experimental data, vol 32. IARC, Lyon, France
Jongeneelen FJ, Leijdekkers CH-M, Bos RP, Theuws JLG, Henderson PTh (1985a) Excretion of 3-hydroxy-benzo(a)pyrene and mutagenicity in rat urine after exposure to benzo(a)pyrene. J Appl Toxicol (in press)
Jongeneelen FJ, Neis JM, Anzion RBM, Roelofs HMJ, Bos RP, Henderson PTh (1985b) 1-Hydroxypyrene, a major metabolite after in vitro incubation of pyrene with human liver preparations. (Submitted)
Keimig SD, Kirby KW, Morgan DP, Keiser JE, Hubert TD (1983) Identification of 1-hydroxypyrene as a major metabolite of pyrene in pig urine. Xenobiotica 13:415–420
Kriebel D, Commoner B, Bollinger D, Bronsdon A, Gold J, Henry J (1983) Detection of occupational exposure to genotoxic agents with urinary mutagen assay. Mutat Res 108:67–79
Møller M, Dybing E (1980) Mutagenicity studies with urine concentrates in the occupational environment. Scand J Work Environ Health 6:216–220
Verhoeve P, Compaan H (1980) Polycyclische aromaten in bet Nederlandse milieu. TNO-MT Reportnr. CL 80/37-122 Delft, the Netherlands (in Dutch)
Wheeler LA, Saperstein MD, Lowe NJ (1981) Mutagenicity of urine from psoriatic patients undergoing treatment with coal tar and ultra-violet light. J Invest Dermatol 77:181–185
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Jongeneelen, F.J., Anzion, R.B.M., Leijdekkers, CM. et al. 1-Hydroxypyrene in human urine after exposure to coal tar and a coal tar derived product. Int. Arch Occup Environ Heath 57, 47–55 (1985). https://doi.org/10.1007/BF00383545
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DOI: https://doi.org/10.1007/BF00383545