(A) Association between previous smoking and CpG sites methylation in F2RL3 in welders and controls (N=228) and (B) Association between F2RL3 methylation and cardiovascular disease by logistic regression (N=228)
| Panel A | ||||||
|---|---|---|---|---|---|---|
| Variable | Unadjusted analysis* | Adjusted analysis† | Full model‡ | |||
| β (95% CI) | p Value | β (95% CI) | p Value | β (95% CI) | p Value | |
| CpG_2 | −1.5 (−2.5 to −0.60) | 0.0014 | −1.5 (−2.5 to −0.55) | 0.0022 | −1.5 (−2.5 to −0.52) | 0.0028 |
| CpG_3 | −0.12 (−0.75 to 0.50) | 0.70 | −0.049 (−0.69 to 0.59) | 0.88 | −0.058 (−0.70 to 0.59) | 0.86 |
| CpG_4 | −4.7 (−6.1 to −3.2) | <0.001 | −4.2 (−5.7 to −2.8) | <0.001 | −4.2 (−5.7 to −2.8) | <0.001 |
| CpG_5 | −2.1 (−3.3 to −0.85) | 0.0010 | −2.0 (−3.2 to −0.69) | 0.0026 | −2.1 (−3.3 to −0.80) | 0.0014 |
| Panel B | ||||
|---|---|---|---|---|
| Variable | Unadjusted analysis§ | Adjusted analysis¶ | ||
| OR (95% CI) | p Value | OR (95% CI) | p Value | |
| CpG_2 | 0.92 (0.84 to 1.001) | 0.054 | 0.93 (0.85 to 1.01) | 0.083 |
| CpG_3 | 0.88 (0.76 to 1.016) | 0.084 | 0.90 (0.77 to 1.04) | 0.14 |
| CpG_4 | 0.96 (0.91 to 1.011) | 0.13 | 0.98 (0.93 to 1.04) | 0.46 |
| CpG_5 | 0.96 (0.89 to 1.024) | 0.20 | 0.97 (0.90 to 1.04) | 0.32 |
The β values are derived from linear regression models.
*Unadjusted analyses: F2RL3 methylation=intercept + β (previous smoking).
†Adjusted analysis: intercept + β (previous smoking) + β1 (age)+ β2 (passive smoking).
‡Full model: intercept + β (previous smoking) + β1 (age) + β2 (passive smoking) + β3 (education) + β4 (current residence).
§Unadjusted analyses: logistic regression, cardiovascular disease=intercept + β (F2RL3 methylation).
¶Adjusted analysis, logistic regression: intercept + β (F2RL3 methylation) + β1 (age)+ β2 (body mass index).