HSL assays used to quantify urinary proteins and enzymes
| π-GST is a relatively new marker for distal tubular damage, superseding Tamm-Horsfall protein excretion.28 | |
| Abbreviations as in table 1. | |
| • | Urinary albumin and transferrin was measured by a latex enhanced, homogeneous competitive immunoassay |
| • | Urinary total protein was measured by a Commassie blue dye binding technique |
| • | The urinary enzymes NAG and LAP were measured kinetically, employing p-nitrophenyl-N-glucosaminide as substrate and standard assays, respectively and using a COBAS FARA (Roche Products, UK) centrifugal analyser |
| • | RBP was measured by an enzyme linked immunosorbent technique |
| • | π- and α-GST were measured by quantitative immunoassays using NEPHKIT-π and NEPHKIT-α (Biotrin International, Dublin, Ireland) kits respectively |