Results of urine mutagenicity using three salmonella tester strains and of DNA damage in lymphocytes
| Genotoxicity assay | Parameters | S1* | S2* | S3* |
|---|---|---|---|---|
| *Time points for biological sampling: S1, the morning the day of captan use; S2, the evening the same day; and S3, the morning the day after. | ||||
| †MSB, mean spontaneous background (SD) between individual experiments. | ||||
| ‡Ratio between the highest number of reverse mutations in urine samples and the spontaneous background of the corresponding experiment. | ||||
| §Mutagenic power calculated as the net His+ colonies/mmol urine creatinine for urines classified as mutagenic according to statistical analysis. | ||||
| ¶Percentage of nuclei ranked as damaged or highly damaged. | ||||
| **HDC, highly damaged cell. | ||||
| ††NT, not tested. | ||||
| Salmonella mutagenicity assay | ||||
| Salmonella strain YG1041 with S9mix [MSB† = 143 (16)] | Mean ratio (SD)‡ | 1.29 (0.17) | 1.42 (0.46) | 1.34 (0.26) |
| [range] | [1.03–1.69] | [1.07–3.55] | [0.95–2.10] | |
| Number of mutagenic urines (%) | 15/27 (56%) | 18/29 (62%) | 17/29 (59%) | |
| Mean mutagenic power§ | 1854 (1262) | 2675 (2470) | 2261 (2038) | |
| [range] | [485–5216] | [538–9623] | [583–7107] | |
| Salmonella strain TA97 without S9mix [MSB† = 164 (15)] | Mean ratio (SD)‡ | 1.04 (0.11) | 1.09 (0.10) | 1.07 (0.11) |
| [range] | [0.84–1.30] | [0.90–1.27] | [0.82–1.40] | |
| Number of mutagenic urines (%) | 2/28 (7%) | 2/29 (7%) | 1/29 (3%) | |
| Mutagenic power | 398 & 2409 | 480 & 1932 | 3209 | |
| Salmonella strain TA102 without S9mix [MSB† = 337 (31)] | Mean ratio (SD)‡ | 1.11 (0.10) | 1.08 (0.09) | 1.07 (0.10) |
| [range] | [0.91–1.37] | [0.93–1.23] | [0.80–1.25] | |
| Number of mutagenic urines (%) | 7/28 (25%) | 5/29 (17%) | 5/29 (17%) | |
| Mean mutagenic power§ | 1612 (1128) | 1465 (1067) | 1602 (980) | |
| [range] | [650–4005] | [701–3321] | [966–3266] | |
| Alkaline comet assay | ||||
| Mean % DNA damage | 10% | NT†† | 13% | |
| [range]¶ | [2–21%] | [5–49%] | ||
| Mean tail moment w/o HDC** (SD) | 4.35 (1.11) | NT | 4.80 (2.57) | |
| [range] | [2.16–5.85] | [3.18–12.76] | ||