Abstract
Objectives
To evaluate allergic sensitisation to Chrysonilia sitophila, Penicillium glabrum, and Trichoderma longibrachiatum in cork workers with asthma.
Methods
Skin prick tests with a battery of common allergens and with the three fungi were performed on ten cork workers with asthma and eight non-exposed asthmatics. Based on serial peak expiratory flow measurements, five were classified as having occupational asthma (AO) and five as having non-occupational asthma (NOA). In exposed patients, specific antibodies for the three fungi were also studied by immunoblotting
Results
Two out of ten patients with occupational exposure and four out of eight of the control group showed positive results for skin prick tests for common allergens. Moreover, two out of five patients with OA and three out of eight controls exhibited sensitisation to storage mites. All exposed patients (with OA or NOA) had negative skin prick test results for the fungal extracts. In patients with asthma and occupational exposure, immunoblotting results confirmed the absence of specific IgE. However, specific IgG4 was present in some cases.
Conclusions
Atopy does not seem to characterise occupational asthma in cork workers. Despite their long exposure to moulds, we could not find evidence of IgE sensitisation to the three most prevalent cork fungi in patients with OA, which points to the search for other causative agents, such as cork chemical compounds or contaminants.
References
Alegre J, Morell F, Cobo E (1990) Respiratory symptoms and pulmonary function of workers exposed to cork dust, toluene diisocyanate and conidia. Scand J Work Environ Health 16:175–181
Alvarez MJ, Castillo R, Rey A, Ortega N, Blanco C, Carrillo T (1999) Occupational asthma in a grain worker due to Lepidoglyphus destructor, assessed by bronchial provocation test and induced sputum. Allergy 54:884–889
Arlian LG, Vyszenski-Moher DL, Johansson SG, van Hage-Hamsten M (1997) Allergenic characterization of Tyrophagus putrescentiae using sera from occupationally exposed farmers. Ann Allergy Asthma Immunol 79:525–529
Blainey AD, Topping MD, Ollier S, Davies RJ (1989) Allergic respiratory disease in grain workers: the role of storage mites. J Allergy Clin Immunol 84:296–303
Cockroft DW, Kilhan DN, Mellon GJA (1977) Bronchial reactivity to inhaled histamine, a method and clinical survey. Clin Allergy 7:235–243
Colloff MJ, Merrett TG, Merrett J, McSharry C, Boyd G (1997) Feather mites are potentially an important source of allergens for pigeon and budgerigar keepers. Clin Exp Allergy 27:60–67
Cote J, Chan H, Brochu G, Chan-Yeung M (1991) Occupational asthma caused by exposure to neurospora in a plywood factory worker. Br J Ind Med 48:279–282
Cullinan P (1998) Occupational asthma, IgE and IgG. Clin Exp Allergy 28:668–670
Danesh P, Velez Caldas F, Figueiredo MarquesJ, San Romão MV (1997) Mycobiota in Portuguese normal and green cork throughout the manufacturing process of stoppers. J Appl Microbiol 82:689–694
Delgado L, Winck JC, Sapage JM, Torres S, Ribeiro JI, Moura e Sá J (1992) Respiratory disease in cork workers: characterization of suberosis alveolitis by bronchoalveolar lavage. Eur Respir J 5 [Suppl 15]:1671
Delgado L, Winck J, Sapage J, Torres S, Moura e Sa J, Fleming Torrinha JA (1995) Antibodies to Penicillium frequentans in cork workers’ respiratory disease (suberosis). Application of the ImmunoCAP IgG RAST in sera and bronchoalveolar lavage (BALF) measurements. Basomba A, Hernandez M, de Rojas F (eds) XVI European Congress of Allergology and Clinical Immunology, Madrid, pp 209–214
Devey M, Panzani ER (1975) The IgG subclasses of antibodies to castor bean allergen in patients with allergic asthma: detection of a high incidence of antibodies to the IgG4 subclass. Clin Allergy 5:353–361
Halpin DM, Graneek BJ, Turner-Warwick M, Newman Taylor AJ (1994) Extrinsic allergic alveolitis and asthma in a sawmill worker: case report and review of the literature. Occup Environ Med 51:160–164
Heederik D, Newman Taylor AJ (1999) Occupational asthma in the baking industry. Asthma in the workplace. Bernstein DI (ed). Marcel Dekker, New York, pp 377–397
Hirakata Y, Katoh T, Ishii Y, Kitamura S, Sugiyama Y (2002) Trichosporon asahii-induced asthma in a family with Japanese summer-type hypersensitivity pneumonitis. Ann Allergy Asthma Immunol 88:335–338
Karjalainen A, Martikainen R, Klaukka T (2002) The risk of asthma among Finnish patients with farmers’ lung. Int Arch Occup Environ Health 75:587–590
Lehrer SB, Hughes JM, Altman LC, Bousquet J, Davies RJ, Gell L, et al (1994) Prevalence of basidiomycete allergy in the USA and Europe and its relationship to allergic respiratory symptoms. Allergy 49:460–465
Licorish K, Novey HS, Kozak P, Fairshter RD, Wilson AF (1985) Role of Alternaria and Penicillium spores in the pathogenesis of asthma. J Allergy Clin Immunol 76:819–825
Luczynska CM, Griffin P, Davies RJ, Topping MD (1990) Prevalence of specific IgE to storage mites (A siro, L destructor and T longior) in an urban population and cross reactivity with house dust mite (D. pteronyssinus). Clin Exp Allergy 20:403–406
Park H-S, Hong C-S (1991) The significance of specific IgG and IgG4 antibodies to a reactive dye in exposed workers. Clin Exp Allergy 21:357–362
Pimentel J, Ávila R (1973) Respiratory disease in cork workers (suberosis) Thorax 28:409–423
Platts-Mills TA, Longbottom J, Edwards J (1987) Occupational asthma and rhinitis related to laboratory rats: serum IgG and IgE antibodies to the rat urinary allergen. J Allergy Clin Immunol 79:505–515
Platts-Mills T A, Vaughan J, Squillace S (2001) Sensitization, asthma, and a modified Th2 response in children exposed to cat allergen: a population-based cross-sectional study. Lancet 357:752–756
Rocha S, Delgadillo I, Ferrer Correia AJ (1996) GC-MS study of volatiles of normal and microbiologically attacked cork from Quercus suber L. J Agric Food Chem 44:865–871
Tarlo SM, Wai Y, Dolovich J, Summerbelt R (1996) Occupational asthma induced by Chrysonilia sitophila in the logging industry. J Allergy Clin Immunol 97:1409–1413
Warren CP, Holford-Strevens V, Sinha RN (1983) Sensitization in a grain handler to the storage mite Lepidoglyphus destructor (Schrank) Ann Allergy 50:30–33
Winck JC, Delgado L, Vanzeller M, Guimaraes T, Torres S, Sapage JM (2001) Monitoring of peak expiratory flow rates in cork workers’ occupational asthma. J Asthma 38:357–362
Winck JC, Delgado L, Vanzeller M, Guimaraes T, Torres S, Sapage JM (2002) Broncho-alveolar inflammation in cork workers’ asthma. Allergy Immunol (Paris) 34:199–203
Yokota K, Yamaguchi K, Takeshita T, Morimoto K (1998) The significance of specific IgG4 antibodies to methyltetrahydrophthalic anhydride in occupationally exposed subjects. Clin Exp Allergy 28:694–701
Zhang K, Mills FC, Saxon A (1994) Switch circles from IL-4-directed epsilon class switching from human B lymphocytes: evidence for direct, sequential, and multiple step sequential switch from mu to epsilon Ig heavy chain gene. J Immunol 152:3427–3435
Acknowledgments
We thank Dr. Miguel Casanova from CBF Leti (Madrid, Spain) for the skin prick test extracts, and Melissa Lopez from DPC (Los Angeles, USA) for immunoblotting preparation.
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Winck, J.C., Delgado, L., Murta, R. et al. Cork workers’ occupational asthma: lack of association with allergic sensitisation to fungi of the work environment. Int Arch Occup Environ Health 77, 296–300 (2004). https://doi.org/10.1007/s00420-003-0494-x
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DOI: https://doi.org/10.1007/s00420-003-0494-x