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Polymorphisms within the putative micro-RNA binding sites of mesothelin gene are associated with the serum levels of the mesothelin-related protein.
  1. Alfonso Cristaudo1,
  2. Rudy Foddis1,
  3. Alessandra Bonotti1,*,
  4. Silvia Simonini1,
  5. Agnese Vivaldi1,
  6. Giovanni Guglielmi1,
  7. Rossella Bruno2,
  8. Debora Landi2,
  9. Federica Gemignani2,
  10. Stefano Landi2
  1. 1 Department of Endocrinology, Orthopaedics, Occupational Medicine, University of Pisa, Italy;
  2. 2 Department of Biology, University of Pisa, Italy
  1. Correspondence to: ALESSANDRA BONOTTI, OCCUPATIONAL MEDICINE, UNIVERSITY HOSPITAL OF PISA, VIA SANTA MARIA, 110, PISA, 56100, Italy; abonotti{at}yahoo.it

Abstract

Serum mesothelin, also known as soluble mesothelin-related protein (SMRP), reportedly shows increased levels in epithelial-type Malignant Pleural Mesothelioma (MPM), but sometimes also arrives at high values in healthy asbestos-exposed subjects. This study is aimed to investigate whether single nucleotide polymorphisms in the 3′untranslated region (3′UTR) of the mesothelin-encoded gene (MSLN) are associated with the SMRP levels measured in serum. The 3′UTR of the mesothelin gene was genotyped in 59 healthy asbestos-exposed subjects, selected on the basis of their SMRP levels. Direct sequencing did not show any new polymorphism, but enabled us to genotype two known SNPs (rs1057147, rs57272256). Differences in the mean values of SMRP in wild-type and variant heterozygote groups were calculated. High levels of SMRP in healthy asbestos-exposed subjects were significantly associated with polymorphism rs1057147 (G < A). Regarding rs57272256, there was no statistically significant difference between wild-type and heterozygote groups. Our study suggests that rs1057147 polymorphism can affect mesothelin expression. Although these data need to be confirmed with a larger number of cases, this study warrants further research in order to better understand the relationship between mesothelin polymorphisms and SMRP.

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