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Circulating LPS in the blood from BioProtein production workers
  1. Liv I B Sikkeland (l.i.b.sikkeland{at}medisin.uio.no)
  1. Centre for Occupational and Environmental Medicine, Rikshospitalet-Radiumhospitalet Medical Center, Norway
    1. Marit Skogstad (marit.skogstad{at}stami.no)
    1. National Institute of Occupational Health, Oslo, Norway
      1. Reidun Øvstebø (reidun.ovstebo{at}medisin.uio.no)
      1. The R&D Group, Department of Clinical Chemistry, Ullevaal University Hospital,Oslo, Norway
        1. Berit Brusletto (b.s.brusletto{at}medisin.uio.no)
        1. The R&D Group, Department of Clinical Chemistry, Ullevaal University Hospital,Oslo, Norway
          1. Kari B F Haug (k.b.f.haug{at}medisin.uio.no)
          1. The R&D Group, Department of Clinical Chemistry, Ullevaal University Hospital,Oslo, Norway
            1. Johny Kongerud (johny.kongerud{at}rikshospitalet.no)
            1. Department of Respiratory Medicine, UiO, Rikshospitalet-Radiumhospitalet Medical Center, Oslo, Norway
              1. Wijnand Eduard (wijnand.eduard{at}stami.no)
              1. National Institute of Occupational Health, Oslo, Norway
                1. Peter Kierulf (peter.kierulf{at}medisin.uio.no)
                1. The R&D Group, Department of Clinical Chemistry, Ullevaal University Hospital,Oslo, Norway

                  Abstract

                  Background: Workers producing bacterial single cell protein (BSCP), BioProtein, are exposed to organic dust containing high levels of endoxins (LPS). Workers in this industry have complained of episodes with fever, fatigue, chest tightness, skin dryness and rubor. The aim of the present study was to quantify LPS and inflammatory mediators in plasma among the workers and non-exposed control subjects. Methods: We included 8 non-smoking production workers, aged 32-51 (median 38), and 8 non-smoking, aged 30-51 (median 39), non-exposed controls. Airborne and plasma endotoxin concentrations were measured, as well as plasma hsCRP and different cytokines, chemokines and metalloproteinases. Results: The workers who did not use personal respiratory protection were exposed to varying airborne levels of endotoxin, 430 (75-15000) EU/m3 (median, range). The level of plasma LPS was significantly elevated (p=0.01) among the workers compared to the non-exposed controls. The workers also had elevated levels of MCP-1 (p=0.02), MIP-1α (p=0.05) and MMP-3 (p=0.04). IL-6 and hsCRP were also elevated among the exposed group, but not significantly (p=0.10 and p=0.07, respectively). Conclusions: In the present study, we detected LPS in plasma of individuals exposed to high levels of LPS at their workplace. This finding is supported by elevated levels of several inflammatory cytokines among the workers, significantly exceeding that of the non-exposed control group. To the best of our knowledge, this is the first time that plasma LPS, together with increased inflammatory markers in plasma, has been detected in an occupational setting.

                  • LPS/endotoxins
                  • bacteria
                  • cytokines
                  • inflammation

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