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Association between plasma BPDE-Alb adduct concentrations and DNA damage of peripheral blood lymphocytes among coke oven workers
  1. Hong Wang
  1. Tongji Med Colloge, HUST, China
    1. Weihong Chen
    1. Tongji Med Colloge, HUST, China
      1. Hongyan Zheng
      1. Tongji Med Colloge, HUST, China
        1. Liang Guo
        1. Taiyuan Steel and Iron Limited Co, China
          1. Huashan Liang
          1. Taiyuan Steel and Iron Limited Co, China
            1. Xiaobo Yang
            1. Tongji Med Colloge, HUST, China
              1. Yun Bai
              1. Tongji Med Colloge, HUST, China
                1. Jianya Sun
                1. Taiyuan Steel and Iron Limited Co, China
                  1. Yougong Su
                  1. Taiyuan Steel and Iron Limited Co, China
                    1. Yongwen Chen
                    1. Tongji Med Colloge, HUST, China
                      1. Jing Yuan
                      1. Tongji Med Colloge, HUST, China
                        1. Yongyi Bi
                        1. Wuhan University, China
                          1. Qingyi Wei
                          1. Tongji Med Colloge, HUST, China
                            1. Tangchun Wu (wut{at}mails.tjmu.edu.cn)
                            1. Tongji Medical College, Huazhong University of Science and Technology, China

                              Abstract

                              Objectives: Coke oven emissions (COEs) containing polycyclic aromatic hydrocarbons (PAHs) can induce both benzo[a]pyrene-r-7, t-8, t-8,c-10-tetrahydotetrol-albumin (BPDE-Alb) adducts and DNA damage. However, the relationship between these biomarkers for early biological effects is not well documented in coke oven workers. Methods: In this study, we recruited 207 male workers exposed to COEs and 102 controls not exposed to COEs in the same steel plant in northern China. We measured BPDE-Alb adduct concentrations in plasma with reverse-phase high-performance liquid chromatography and DNA damage in peripheral blood lymphocytes with alkaline comet assay. Results: Our results showed that the median concentration of BPDE-Alb adducts in exposed group (34.36 fmol/mg albumin) was significantly higher than that in control group (21.90 fmol/mg albumin, P=0.012). The mean Olive tail moment (Olive TM) of DNA damage in exposed and control groups were 1.20 and 0.63, respectively (P=0.000). Multivariate logistic regression analysis revealed that the odds ratio (OR) for BPDE-Alb adduct and Olive TM associated with the exposure were 1.72 [95% confidence interval (CI) =1.06-2.81] and 1.96 (95%=1.20-3.19), respectively. Our results showed that there were significant correlations between the concentrations of BPDE-Alb adduct and Olive TM levels in exposed group (rs=0.235, P=0.001) but not in control group (rs=0.093, P=0.353). Conclusion: Our results suggested that occupational exposure to COEs may induce both BPDE-Alb adducts and DNA damage in lymphocytes of the coke oven worker and that these two markers are useful for monitoring exposure to COEs at work place.

                              • 7,8-dihydroxy-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene
                              • DNA damage
                              • albumin adduct
                              • biomarkers
                              • coke oven emissions

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