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Original Article
A cohort mortality study of lead-exposed workers in the USA, Finland and the UK
  1. Kyle Steenland1,
  2. Vaughn Barry1,
  3. Ahti Anttila2,
  4. Markku Sallmén3,
  5. Damien McElvenny4,
  6. AC Todd5,
  7. Kurt Straif6
  1. 1 Rollins School PubHealth, Emory University, Atlanta, USA
  2. 2 Finnish Cancer Registry, Helsinki, Finland
  3. 3 Finnish Institute of Occupational Health, Work, Environment, Helsinki, Finland
  4. 4 Insitute of Occupational Medicine, Edinburgh, UK
  5. 5 Icahn School of Medicine at Mount Sinai, New York, USA
  6. 6 International Agency for Research on Cancer (IARC), Lyon, France
  1. Correspondence to Dr Kyle Steenland, Rollins School PubHealth, Emory University, Atlanta 30322, USA; nsteenl{at}emory.edu

Abstract

Objectives To investigate further whether inorganic lead is a carcinogen among adults, or associated with increased blood pressure and kidney damage, via a large mortality study.

Methods We conducted internal analyses via Cox regression of mortality in three cohorts of lead-exposed workers with blood lead (BL) data (USA, Finland, UK), including over 88 000 workers and over 14 000 deaths. Our exposure metric was maximum BL. We also conducted external analyses using country-specific background rates.

Results The combined cohort had a median BL of 26 µg/dL, a mean first-year BL test of 1990 and was 96% male. Fifty per cent had more than one BL test (mean 7). Significant (p<0.05) positive trends, using the log of each worker’s maximum BL, were found for lung cancer, chronic obstructive pulmonary disease (COPD), stroke and heart disease, while borderline significant trends (0.05≤p≤0.10) were found for bladder cancer, brain cancer and larynx cancer. Most results were consistent across all three cohorts. In external comparisons, we found significantly elevated SMRs for those with BLs>40 µg/dL; for bladder, lung and larynx cancer; and for COPD. In a small subsample of the US cohort (n=115) who were interviewed, we found no association between smoking and BL.

Conclusions We found strong positive mortality trends, with increasing BL level, for several outcomes in internal analysis. Many of these outcomes are associated with smoking, for which we had no data. A borderline trend was found for brain cancer, not associated with smoking.

  • lead
  • cancer
  • occupation

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What this paper adds

  • Inorganic lead is considered a probable carcinogen by the International Agency for Research on Cancer (IARC), and adult lead exposure is known to raise blood pressure, and suspected of increasing rates of heart disease and stroke.

  • We have analyzed mortality among 88 000 workers with data on blood lead levels in three countries, with 14 000 deaths, and a median maximum blood lead of 26 µg/dL.

  • Significant(p<0.05) positive trends were found between blood lead levels and lung cancer, chronic obstructive pulmonary disease (COPD), stroke and heart disease, whereas borderline significant trends (0.05<=p<=0.10) were found for bladder cancer, brain cancer and larynx cancer. Many of these outcomes are associated with smoking, for which we had little or no data. In a small sub sample of the US cohort (n=115) who were interviewed, we found no association between smoking and maximum blood lead. On theoretical and a priori grounds, we do not think it is likely that confounding by smoking can explain our positive findings.

Introduction

With the worldwide elimination of leaded gasoline, lead levels have dropped considerably in the environment and in the blood of the world population.1 2 Nonetheless, lead remains a relatively common environmental and occupational exposure. For example, the US OSHA estimates that approximately 804 000 workers in general industry, and an additional 838 000 workers in construction, are currently potentially exposed to lead (https://www.osha.gov/SLTC/lead/). This represents about 2% of the male workforce; most exposed workers are male (in 2012, the National Institute of Occupational Safety and Health (NIOSH) estimated that 92% of workers with blood lead (BL) levels above 10 µg/dL, ie, occupational levels, were male, see https://www.cdc.gov/mmwr/preview/mmwrhtml/mm6254a4.htm?s_cid=mm6254a4_w). However, this may be an underestimate of current exposure prevalence, and is likely to underestimate the prevalence of lead exposure in the past. The last systematic survey of US workers took place in the 1980s. At that time, the NIOSH estimated that more than 3 million workers in the USA were potentially exposed to lead,3 representing at the time about 5% of the male workforce.

Both the International Agency for Research on Cancer (IARC) and the National Toxicology Program have concluded that inorganic lead is a probable human carcinogen, based primarily on evidence of the impacts of lead exposure on lung and stomach cancers and some suggestion of an effect on brain cancer.4 5

Lead exposure has been associated with modest increases in blood pressure, which may increase risk of stroke or heart disease. A meta-analysis of 31 studies by Nawrot et al 6 found that most showed a positive association between BL and blood pressure after controlling for age: a doubling of BL was associated with a 1.0 mm rise in systolic pressure (95% CI 0.5 to 1.4) and a 0.6 mm Hg increase in diastolic pressure (95% CI 0.4 to 0.8). Increased blood pressure is a risk factor for stroke and heart disease, but information on these outcomes in relation to lead exposure is limited in the current literature. In a review of articles concerning lead and the risk of cardiovascular disease (CVD), Navas-Acien et al 7 found that overall there was insufficient epidemiological data to draw conclusions. A subsequent study of 868 US men in the Normative Aging Study,8 with past exposure to background lead levels, found strong positive trends between baseline bone lead and CVD but not baseline BL. Navas-Acien et al 9 found a relationship between bone lead and increased blood pressure. These findings suggested that the cumulative exposure to higher blood levels in the past, reflected by the bone lead, might have a damaging cardiovascular effect not reflected by short-term exposure at baseline as assessed by BL. The Global Burden of Disease 201310 has used (1) the estimated BL and bone lead across countries, (2) the relationship between bone lead and blood pressure from,9 and (3) the estimated relationship between blood pressure and both heart disease and stroke, to estimate a burden of disease from lead. They have estimated that BL levels above background are responsible for 850 000 deaths, due to increased stroke and heart disease stemming from increased blood pressure.10 More detailed global burden of disease (GBD) analyses of stroke indicated that 7% of stroke worldwide was attributable to BL levels above 20 µg/dL.11

Lead exposure is also associated with kidney disease. A comprehensive review of lead-related nephrotoxicity concluded that lead contributes to nephrotoxicity, even at BL levels below 5 µg/dL, especially in people with other illnesses such as hypertension and diabetes.12 However, occupational mortality studies of lead-exposed workers have not consistently shown associations with kidney disease, which is a relatively rare cause of death.

To further investigate these associations, we have conducted a pooled analysis for mortality among three large cohort of workers with documented BL levels (n=88 000). These workers were enrolled in surveillance programmes in the USA, the UK and Finland, which measured inorganic lead in their blood. The pooled analysis allows increased power to observe exposure–outcome relationships. Furthermore, follow-up of the Finnish cohort has been extended 25 years, and the results have not been previously published. The documented BL levels among these workers provide data on body burden, which avoids some of the uncertainty of whether workers were actually exposed to lead, which occurs when exposure is estimated based on job title or a job-exposure matrix.

Methods

Cohorts

The US cohort of 58 000 male workers was assembled from the Adult Blood Lead Surveillance (ABLES) programme, sponsored by NIOSH. ABLES started collecting state-level data on BL levels in 1987, starting with 4 states and increasing up to 41 states in 2012.13 States monitored testing laboratories to collect their data. Initially states collected data only on those with≥25 µg/mL, but over time some states began to collect data on lower levels. BL levels were measured at different times in different states, but generally started in the early 1990s and went through 2007. Data on industry were collected in only some states, for those with≥25 µg/dL blood levels, amounting to about 10% of ABLES subjects. Among those, 62% were in manufacturing, 10% in construction, 7% in metal mining, 1% in scrap metal and 20% in other industries. We included data from 11 states which had the majority of the ABLES data. We conducted mortality follow-up through 2010. Results of this follow-up have been published.14

The Finnish cohort of 21 000 workers (12% women) was created from those with documented BL in the period 1973–1983, based on laboratory reports and employer data. Finnish labour law mandated that if the BL of any worker in the workplace exceeded 42 µg/dL, then all workers in that workplace should have their BL measured. Those with the most blood tests came from the battery industry, lead smelting, metal foundries, railroad machine shops and chemical factories. Those monitored for non-occupational reasons were excluded. The cohort was originally followed through 1988.15 16 Follow-up has now been extended through 2013.

The UK cohort numbered 9000 (15% women), and has been followed through 2011. Results of follow-up have been recently published.17 The cohort had BL levels measured in the period 1975–1979, as part of an effort at that time to conduct a census of all workers exposed to lead. The principal industrial sectors included pottery/glaze (14%), lead battery (12%), lead smelting (10%) and demolition/scrap metal (9%).

Cohort members were exposed in a large number of different occupations and industries in each country. The subjects in these cohorts were exposed almost exclusively occupationally, although in the US cohort there were a small number of men exposed recreationally in firing ranges.

Exposure data

The exposure data consisted of BL tests, conducted under occupational surveillance programmes. Details are available in the original publications.14–17 Complete work histories were not available, nor were data on any demographic variables beside birth date and gender. Half of the combined cohort had only one BL test, while the other half with more than one test had a median of four tests. For descriptive data, we report means when the data are normally distributed, and medians (or both) otherwise.

When a worker had multiple blood measurements, these were available for the USA and Finland, but for the UK we had only the maximum, the minimum and the number of measurements. We used the maximum BL as the principal exposure metric. This choice was motivated by two factors. One, generally when more than one BL measurement was available, they tended to be similar to each other, so the maximum was not far from the mean (see data below). Second, absent any accurate measure of cumulative exposure, we believed that the maximum BL was likely to be a reasonable choice for the most biologically relevant measure to predict future disease risk.

We calculated the intraclass correlation coefficient for workers with more than one blood test, that is, the proportion of the variance between workers, out of the total variance (the sum of between and within variances). For the UK, in which we did not have data on all BL measurements, but only the mean, the maximum and the number of measurements, we estimated the variance of the mean, for each worker with multiple tests, by (1) estimating the log(minimum) assuming symmetry with log(max), and (2) using the range/4 of logged values as an estimate of the SD on log scale, then (3) converting to original unlogged scale.18

Here we have assumed that BL measurements were reasonably comparable across countries. However, there is some uncertainty here. A panel of industrial hygienists analysing this question was unable to reach a definitive conclusion about this point,19 and recommended inclusion of a variable for ‘country’ be included in analyses, which we have done.

Mortality analyses

Person-time began at time of first BL test, and continued until the end of follow-up, death or year of emigration. We conducted internal analyses for different mortality outcomes via Cox regression (SAS PHREG, V. 9.22, http://support.sas.com/en/support-home.html), with age as the time variable, while controlling for decade of year of birth, gender and country, and using the full risk set. Definition of different disease categories was made according to the groupings used by the NIOSH life table (see footnotes to tables 3 and 4).20 21 We tested the proportional hazard assumption for exposure (equivalent to testing an interaction between exposure (BL level) and age), which was not violated. We also tested for interaction between BL level and country. We did not conduct lagged analyses as the true time of first exposure was unknown. We conducted both categorical analyses and continuous analyses. Categorical analyses used <20 µg/dL maximum BL as the referent, and included categories 20–29, 30–39 and 40+ µg/dL. The UK data had only 1% of subjects with <10 µg/dL, motivating the use of <20 µg/dL as the referent. Furthermore, several authors have recently called for lowering currently permissible occupational BL levels (40 µg/dL in many countries) to 20 µg/dL, that is, under the assumption that the latter level is a reasonably safe level.22 23 The American College of Occupational and Environmental Medicine recently called for removal of workers from exposure when their BL levels exceed 20 µg/dL.2 Currently, the US Occupational Safety and Health Administration requires removal of workers from exposure only when BL levels exceed 50 µg/dL (construction) or 60 µg/dL (general industry), and allows them to return only when their BL drops below 40 µg/dL (https://www.osha.gov/pls/oshaweb/owadisp.show_document?p_id=10033&p_table=STANDARDS).

For two countries where we had data on multiple tests per person (Finland and USA), we also ran analyses using time-dependent maximum BL. Results were virtually identical for these two countries to analyses using time-independent maximum BL. Therefore, for simplicity, and given that only time-independent maximum BL was available for the UK, we used time-independent maximum BL for all analyses. We tested for continuous trends using either maximum BL or its natural log. As the log term tended generally to fit the data better, we report trend tests using only the log of maximum BL.

We conducted external analyses via SMRs, using country-specific national mortality rates, stratified by 5-year age and calendar time categories, using the NIOSH Life Table Analysis System.20 Again, we used the International Classification of Disease (ICD) groupings for different mortality outcomes from the NIOSH life table, to create disease-specific external rates.21

Cross-sectional data on bone lead

We have studied tibia lead in 115 US cohort members, as a way to determine whether maximum BL in the past is correlated with current bone lead, the latter being a measure of cumulative absorbed dose. Cohort members living near New York City were invited to undergo a tibia lead measurement, conducted at Icahn School of Medicine at Mount Sinai University by A.C. Todd, PhD. The study is designed to assess bone lead among 200 US cohort members; we report here preliminary analyses of the first 115 tested. In addition to bone lead, we report on smoking habits of the men tested.

K-shell X-ray fluorescence has been used to measure lead in bone in numerous studies over the past 40 years. The particular method we used has been described by Todd et al,24 and shown to be repeatable in the field.25 The procedure takes about 30 min. In brief, the 88.034 keV γ-rays from 109Cd are used to fluoresce the K-shell X-rays of lead. The 109Cd γ-rays can also undergo elastic scatter off an atom of primarily calcium and phosphorus in bone. The γ-rays that escape the body after they have interacted, and which are head in the direction of the radiation detector, can be recorded by the spectroscopy system which yields an energy distribution, or spectrum (number of photons of energy that lies in a particular energy range vs the mid-point of that energy range) of the recorded photons. The spectrum is then ‘fitted’ using a non-linear, least-squares technique with a mathematical function to extract the amplitudes of the X-ray and elastic scatter peaks that rise above the background continuum of scattered photons. The ratio of the X-ray to elastic peaks is the response of the system and is regressed, for each X-ray peak under analysis, against the lead concentration of the calibration standards to produce a calibration line. The signal from a subject measured in vivo is compared with the established calibration line for each lead X-ray to be analysed to obtain one or more estimates of the subject’s bone lead concentration

Results

Table 1 provides a summary of cohort statistics. The US cohort was assembled later and was considerably younger than the other two cohorts.

Table 1

Descriptive data for three cohorts

Table 2 provides summary data on the BL tests, including some data on those with multiple BL measurements (50% of the combined cohort). The intraclass correlation coefficient (proportion of variance between subjects out of total variance) for those with multiple measurements was 0.86, indicating that the variance with a person’s measurement was much less than the variance between the means of different workers.

Table 2

Distribution of blood lead levels

Those in higher maximum BL category had somewhat longer time since first BL test; this time increased from 17 to 22 years from the lowest to higher category.  They had an earlier first BL test (1988 for those >40 µg/dL vs 1993 for those with <20 µg/dL) and more BL tests (increasing trend, median 1.5 tests for those in the <20 µg/dL category, 4.0 tests for those in the >40 µg/dL category). They were also born somewhat earlier (mean 1953 for <20 µg/dL, mean 1948 for those in the >40 µg/dL category).

Table 3 gives the results for selected non-malignant causes of a priori interest (all deaths, heart disease, stroke, chronic obstructive pulmonary disease (COPD) and kidney disease). There are positive statistically significant trends for all these, except non-malignant kidney disease where the uppermost category showed a 54% excess, but this finding was limited by small numbers. Statistically significant trends are driven by both the increased trends in HRs and the large numbers of deaths for these causes.

Table 3

Categorical and continuous results of mortality analyses by blood lead category for selected non-malignant causes of death

For cancers, we tested for trend for 13 malignant cancers with appreciable numbers (bladder, brain (benign included), breast (women only), oesophageal, kidney, larynx, lung, pharynx, stomach, leukaemia, Hodgkin’s disease, multiple myeloma and non-Hodgkin’s lymphoma). All trends were positive (increasing rate with increasing BL), although not necessarily statistically significant, with the exception of stomach cancer and leukaemia. Table 4 shows the categorical  results and trend tests for four cancers for which the p value for positive trend was <0.10, two of which were of a priori interest (lung, brain), and two of which were not (bladder, larynx). Table 4 also shows data for two cancers of a priori interest which showed little trend; stomach cancer (p for trend 0.93, 195 deaths) and kidney cancer (p for trend 0.79, 128 deaths).

Table 4

Categorical and continuous results of mortality by blood lead category for selected cancers*

There were generally few interactions by country. For outcomes with positive trends in the no-interaction models, we subsequently found significant interactions for lung cancer (UK positive trend, p=0.14; USA/Finland positive trends, p<0.0001) and stroke (USA positive trend, p=0.13; UK/Finland trends, p=0.0002 and p=0.0009, respectively).

Results for SMRs for all three countries combined (using national rates as the referent) are shown in table 5, for causes for which rates were available across all three countries. Rates were available from time of first blood testing in all countries. For those in thie highest BL category, there are significant (p<0.05) excesses for bladder cancer, lung cancer, COPD and larynx cancer, and deficits for kidney cancer, multiple myeloma and leukaemia. There was no excess for brain cancer, an a priori outcome of interest, based only on malignant cases (external rates were not available for benign brain cancer). Deaths from transportation accidents are linked to lower socioeconomic status (SES),26 but did not show an excess versus the general population.

Table 5

SMRs for cohort members by maximum blood lead category*

In the cross-sectional analyses of the subsample of US cohort members (n=115), the average age was 61 (range 39–85), 85% were white, 14% Hispanic and 40% had a high school education or less. The smoking habits were as follows: current smokers (14%), former smokers (44%) and never smokers (42%). Maximum BL was distributed as follows: 33% <20 µg/dL, 42% 20–39 µg/dL and 25% >40 µg/dL. Smoking history measured via pack-years was not correlated with maximum BL (Spearman correlation r=0.01). Ever smokers and never smokers did not differ with respect to their maximum bone lead (p=0.80). Linear regression analyses controlling for age, ethnicity and education did not change these null results. In linear regression analyses controlling for age, ethnicity and education, maximum BL was a strong positive predictor of bone lead (p=0.004)(model R-square 0.29). However, this positive trend was not monotonic, but driven by those with maximum BL≥40 µg/dL. In analyses using quartile of maximum BL, the estimated change in bone lead for the second, third and fourth quartiles of BL versus the first was respectively −1.7 (p=0.47), −3.6 (p=0.16) and +8.3 (p=0.004)(R-squared model 0.40).

Discussion

Our study was based on workers with lead exposure documented via BL tests. BL reflects short-term exposure, generally within the last few months.27 This cohort definition has both advantages and disadvantages. Without work history, we do not have a data on first exposure or length of work in a job potentially exposed to lead, making impossible analyses by duration or latency, or lagged exposure. On the other hand, for each individual worker we avoid the uncertainty arising from job-exposure matrices, regarding whether workers in specific jobs were actually exposed to lead, by having a measure of an internal dose.

Bone lead analyses on a subsample of the US cohort found that maximum BL was correlated with bone lead, the latter being a measure of cumulative dose, while the former represents exposure in the recent past (weeks to months). This correlation, however, was driven by those with the highest maximum BL levels, above 40 µg/dL. This may reflect that those in the jobs with the greatest exposure intensity (as measured by BL) experienced the greatest cumulative exposure (as measured by the product of exposure intensity and exposure duration: bone lead).28 Data collection in this cross-sectional study is still underway and a larger sample size may shed further light on this question.

Strong positive trends were found in internal analyses for all causes, lung cancer, bladder cancer, larynx cancer, COPD, heart disease and stroke. A weak non-monotonic trend was found for brain cancer.

Regarding cancer, these findings for cancer are concordant with IARCs 2006 determination4 that lung and brain cancer were two cancers more strongly associated with inorganic lead, but are not concordant regarding stomach cancer. Two studies since the IARC evaluation are worth nothing. Gwini et al 29 studied cancer incidence among male Australian workers in a lead surveillance programme with a similar design to ours. There were 240 incident cancers; the only significant excesses were for liver and oesophageal cancers. However, only 95 of the 240 subjects with cancer had BL levels; of these 95, only 27 had levels at or above 30 µg/dL. Hence, there is little information about specific cancer sites among men with high exposure in this cohort. Liao et al 30 studied cancer incidence among approximately 7000 lead-exposed men and women workers in two plants in Shanghai, comparing them with a large number of unexposed workers, and using a job-exposure matrix to classify workers into none, low, medium and high exposure to either lead dust or fumes. Overall, there were suggestions of an excess among exposed versus non-exposed workers for brain cancer (rate ratio (RR) 1.8, 0.704.28, 10 exposed cases) and kidney cancer (RR 1.4, (0.9–2.3), 17 exposed cases). Borderline significant exceses were found in high-exposed males only for lung and stomach cancer. Overall, our cancer findings are reasonably concordant with other findings for lung and brain cancer, but not for bladder and larynx cancer. The latter is rare and often not reported in occupational lead studies.

All of the outcomes we found to be elevated with higher exposure, with the exception of brain cancer,31 are associated with smoking, which raises the question of whether smoking habits confounded these internal analyses; we had no data on smoking. However, internal analyses of comparing workers with workers are generally less subject to confounding by smoking, compared with SMR analyses comparing workers with the general population.32 In particular, internal comparisons in which the uppermost category showed HRs of 1.8–2.7 (bladder, lung, larynx, bladder, COPD) are unlikely to be due to confounding by smoking under hypothetical or observed smoking differences between low and high-exposed workers. Earlier work has shown that for diseases which are strongly related to smoking (eg, lung cancer), observed smoking differences between low and high-exposed workers are likely to account for excesses of only 20%–40% due to confounding.33 34 In addition, other outcomes strongly associated with smoking, such as oesophageal cancer (trend test p=0.17, 138 deaths), or moderately associated, such as kidney cancer (trend test p=0.79, 128 deaths), did not show significantly positive trends. It might also be noted that in an earlier nested case–control study of lung cancer in the Finnish cohort, a study which had smoking data, smoking was not associated with BL level.16 Finally, in our subsample of US cohort members, we found no association between smoking habits and maximum BL levels.

Smoking is correlated with SES, which is in itself highly related to most causes of mortality. We had census-based data on SES from one cohort. We matched the Finnish data to census data to determine broad occupational category. Matching the cohort to the censuses of 1970, 1975, 1980, 1985 and 1990, 99% of cohort had at least one match. Taking data from the last available census for each worker, the percentage of workers in the blue-collar category by category of BL (ie <10, 10–20, 20–30, 30–40 and 40+ µg/dL) were 71%, 77%, 78%, 78% and 81%, respectively, indicating some, but not dramatic, decrease in SES with higher lead exposure.

Confounding by other occupational exposures in the workplace is another possibility. Regarding occupational carcinogens, workers in our cohort were exposed to lead in many different jobs with many different possible co-exposures, making it less likely that any specific co-exposure to a carcinogen would be responsible for our observed associations with several cancers. On the other hand, dust exposure was likely to have been common to many lead-exposed jobs, and may have contributed to the COPD findings. We know of no prior evidence that lead exposure is associated with COPD. Data on such co-exposures were not available across all cohorts (for further discussion of this issue, see19).

On the other hand, despite potential confounding, there are some arguments for a causal role in lead for many of the outcomes for which we found associations. Other heavy metals are well known to cause lung cancer (nickel, chromium, cadmium, beryllium). Stroke and heart disease were a priori outcomes of interest due to lead’s effect on blood pressure. Brain cancer was an outcome of a priori interest, given the prior findings for brain cancer incidence from the Finnish cohort,15 based on 26 incident cases with follow-up to 1990. However, we know of no a priori reason to associate larynx cancer to metal exposure; occupational exposures associated with larynx cancer include asbestos, sulfuric acid and polycyclic aromatic hydrocarbons (PAHs).35 36

Non-malignant kidney disease, an outcome of a priori interest, did not show a significant positive trend with lead, although did show a 54% (HR 1.54, 0.77–3.08) elevation in the highest category (≥40 µg/dL). Non-malignant kidney disease may be underascertained using underlying cause of death certificates (without reference to contributing causes), reducing power; one study in the USA showed that non-malignant kidney disease was four times more likely to appear as a contributing causes rather than an underlying cause.37 It should be noted that incident end-stage renal disease, in a separate analysis of the American cohort,38 showed an excess of 43% in the highest category (≥51 µg/dL, HR 1.43 (1.01–1.85)), for those with at least 5 years of follow-up.

External comparison analysis via SMRs, among those in   the highest category (≥40 µg/dL), showed that a number of the excesses seen in internal analyses were also observed in comparison with the general population, i.e., for COPD, bladder cancer, larynx cancer and lung cancer. These are the outcomes which had the most pronounced trends in internal analyses. Other outcomes with positive trends in internal analyses, such as heart disease and stroke, may have been more affected by the healthy worker effect in the SMR analyses, occurring when comparing workers to the general population. The US cohort is young and particularly subject to the healthy worker effect, and is bigger than the combined size of the older Finnish and UK cohorts.

One main limitation to our data was our reliance on BL, without a complete work history of lead-exposed jobs, precluding analyses by duration or latency. A second major limitation was the limited number of BL measurements, again precluding a complete picture of cumulative internal exposure. At the same time, the availability of BL for all cohort members was a strength, because we had documentation of lead exposure, and a reasonable way to rank subjects by level of exposure—under the assumption that the range of BLs for a given worker reflected their average exposure level. The other main strength of our data is the large sample size, permitting analyses of relatively rare outcomes.

In summary, findings from this cohort are suggestive of lead effects on several causes of mortality. Confounding by smoking and SES may be playing a role in some of these excesses, but are unlikely to explain them entirely. Planned future analyses of cancer incidence in these cohorts should provide additional information.

References

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Footnotes

  • Contributors All contributors meet the criteria for authorship.

  • Funding Steenland worked on this project as part of his stay at IARC as a Visiting Scientist inthe summers of 2014 and 2015.

  • Competing interests None declared.

  • Ethics approval Emory University IRB: note, this is a record based study with no contact with study subjects.

  • Provenance and peer review Not commissioned; externally peer reviewed.

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