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P081 The difference between plasma and urinary 8-hydroxy-2- deoxyguanosine biomarkers measured by liquid chromatrography tandem mass spectrometry
  1. Chung-Ching Wang1,
  2. Wei-Liang Chen1,
  3. Saou-Hsing Liou2
  1. 1Tri-Service General Hospital, National Defense Medical Centre, Taipei, Taiwan, R.O.C
  2. 2Division of Environmental Health and Occupational Medicine, National Health Research Institutes, Miaoli, Taiwan, R.O.C

Abstract

Although 8-hydroxy-2-deoxyguanosine (8-OHdG) is a widely used promising biomarker of DNA damage, there are concerns about which tissues or body fluids should be sampled. The objective of this study is to evaluate the correlation of DNA oxidative damage biomarkers, 8-OHdG, between blood and urine and risk factors associated with 8 OHdG. The study population was recruited from a baseline survey of a worksite lifestyle study including 96 office workers aged 23 to 60 years. A self-administered questionnaire was used to collect information on personal characteristics. The plasma and urinary 8-OHdG was measured by liquid chromatography tandem mass spectrometry (LC-MS/MS). In linear regression, a positive relation was found (t = 2.3, p < 0.05) between the log-transformed plasma and urinary 8-OHdG levels adjusted for gender, age, BMI, and smoking status. Our findings showed that age, gender and smoking were significantly associated with plasma 8-OHdG, but not with urinary 8-OHdG. Our results suggest that there is a positive relation between the biomarkers of plasma (steady state DNA damage) and urinary 8-OHdG (total DNA damage). However, the plasma 8-OHdG is more sensitive than urinary 8-OHdG to detect increased oxidative damages induced by risk factors.

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