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0032 Polymorphonuclear leukocytes phagocytic capacity in workers occupationally exposed to benzene
  1. Luis Haro-García1,
  2. Kendy Peggy Wek-Rodríguez2,
  3. Carmina Jiménez-Ramírez3,
  4. Cuauhtémoc Arturo Juárez-Pérez3,
  5. Guadalupe Aguilar-Madrid3,
  6. Alfonso Zárate-Amador3,
  7. Oscar Rojas-Espinosa2
  1. 1Universidad Nacional Autónoma de México, FM Salud Pública, Ciudad de México, Mexico
  2. 2Instituto Politécnico Nacional ENCB, Ciudad de México, Mexico
  3. 3Instituto Mexicano Del Seguro Social, UIST, Ciudad de México, Mexico

Abstract

Objectives To determine phagocytic capacity of PMN leukocytes in workers occupationally exposed to benzene.

Method Cross-sectional study that included 54 workers of a paint manufacture company in Mexico City; exposure to benzene was determined through S-phenylmercapturic acid (SPMA) presence in urine. The PMN phagocytic capacity analysis included three parameters: 1) nitro-blue tetrazolium (NBT) reduction, 2) hydrogen peroxide (H2O2) production, and 3) cell adhesion (CAD)

Results In the whole of workers included in the study, NBT reduction = 0.419 ± 0.075, H2O2 production = 6.7 ± 1.4 ng, and CAD = 58.3 ± 6.2 μg. SPMA was identified in all workers although 24 of them are not in occupationally exposure to organic solvents (2.3 ± 0.81 μmol/mol creatinine), while the remaining 30 handle these substances (3.2 ± 1.8, p = 0.02). Among these exposure groups, there were not statistically differences in any of the parameters analysed. Although the simple regression analysis of these parameters with the concentration of SPMA identified in urine, a decrease was observed in NBT reduction (β= -0.009, R2= 0.01), in H2O2 production (β= -0.16, R2= 0.02), and in CAD (β= -0.53, R2= 0.01), none was statistically significant (p ≥ 0.05)

Conclusions PMN phagocytic capacity in the workers studied seems to be intact. Attract attention the consistently decrease of the three parameters in relation to the concentration of SPMA identified in urine even when there was no statistical significance. Some limitations do not allow a more complete analysis, so it is encouraged to make further studies.

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