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Original article
Airborne cultivable microflora and microbial transfer in farm buildings and rural dwellings
  1. Anne-Cécile Normand1,
  2. Bertrand Sudre2,
  3. Mallory Vacheyrou2,
  4. Martin Depner3,
  5. Inge M Wouters4,
  6. Ilka Noss4,
  7. Dick Heederik4,
  8. Anne Hyvärinen5,
  9. Jon Genuneit6,
  10. Charlotte Braun-Fahrländer7,
  11. Erika von Mutius3,
  12. Renaud Piarroux1,
  13. the GABRIEL-A Study Group
  1. 1Department of Parasitology and Mycology, Assistance Publique-Hôpitaux de Marseille, Marseille, France
  2. 2Franche-Comté University, UMR-CNRS 6249 Chrono-Environnement, Mycology Department, Besançon, France
  3. 3Children's Hospital of the University of Munich, Lindwurmstrasse 4, Munich, Germany
  4. 4Institute for Risk Assessment Sciences, Division of Environmental Epidemiology, Utrecht University, TD Utrecht, The Netherlands
  5. 5National Institute for Health and Welfare, Department of Environmental Health, Kuopio, Finland
  6. 6Institute of Epidemiology, University of Ulm, Ulm, Germany
  7. 7Swiss Tropical and Public Health Institute, Basel, Switzerland
  1. Correspondence to Anne-Cécile Normand, Laboratoire de Parasitologie/Mycologie, Hôpital de la Timone, 264 rue Saint-Pierre, 13385 Marseille cedex 05, France; acecilenor{at}hotmail.com

Abstract

Objectives Exposure to environments rich in microorganisms such as farms has been shown to protect against the development of childhood asthma and allergies. However, it remains unclear where, and how, farm and other rural children are exposed to microbes. Furthermore, the composition of the microbial flora is poorly characterised. We tested the hypothesis that farm children are exposed indoors to substantial levels of viable microbes originating from animal sheds and barns. We also expected that environmental microbial flora on farms and in farm homes would be more complex than in the homes of rural control children.

Methods Dust samples were collected using passive samplers in the bedrooms of the following groups of children in rural Bavaria, Germany: (i) those living on farms (n=144), (ii) those regularly exposed to farm environments but not living on farms (n=149) and (iii) those never visiting farms (n=150). For farm children, additional samples were collected in animal sheds and barns. All samples were subjected to fungal and bacterial culturing.

Results Detectable levels of microorganisms were more often found in samples taken from farm dwellings than from other homes. Farm dwellings also showed higher microbial levels. Microbial counts of farm dwelling samples correlated with the counts in corresponding animal sheds and barns.

Conclusions Microorganisms are transported from animal sheds and barns into farm dwellings. Therefore, children living in these environments are exposed when indoors and when visiting animal sheds and barns. Indoor exposure may also contribute to the protective effect of the farm environment.

  • Microbial exposure
  • farming environment
  • fungi
  • bacteria
  • airborne dust
  • aerosols
  • dusts
  • fungi/moulds
  • indoor air

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Footnotes

  • The GABRIEL-A Study Group consists of the following members of GABRIEL-A study centres: Austria: Katalin Kovacs, Bernhard Morass, Elisabeth Horak; Baden-Württemberg (Germany): Gisela Büchele, Nikolaos Sitaridis, Jon Genuneit; Bavaria (Germany): Juliane Weber, Erika von Mutius; Poland: Anna Debinska, Hanna Danielewicz, Barbara Sozanska; Switzerland: Marco Waser, Charlotte Braun-Fahrländer.

  • Funding This work was supported by the European Commission as part of GABRIEL (a multidisciplinary study to identify the genetic and environmental causes of asthma in the European Community), contract number 018996 under the FP6-LIFESCIHEALTH Integrated Program LSH-2004-1.2.5-1.

  • Competing interests None.

  • Provenance and peer review Not commissioned; externally peer reviewed.

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